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PKM antibody (AA 2-531)

Name: PKM antibody (AA 2-531)
Brand: antibodies-online
SKU: ABIN7166916
Price: 385 USD
Availability: InStock

PKM Reactivity: Human ELISA, WB, IHC, IF, IP, FACS Host: Mouse Monoclonal 6C3C7 unconjugated

Western Blot Positive WB detected in: A549 whole cell lysate, 293T whole cell lysate, U251 whole cell lysate, Rat Brain tissue, Rabbit Skeletal Muscle tissue All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1 min (PKM antibody (AA 2-531))

IHC image of ABIN7166916 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (PKM antibody (AA 2-531))

IHC

Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1 min (PKM antibody (AA 2-531))

Western Blot Positive WB detected in: Mouse Heart tissue, Mouse Brain tissue, Mouse Skeletal Muscle tissue All lanes: PKM antibody at 1:4000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 55-60 kDa Observed band size: 55-60 kDa (PKM antibody (AA 2-531))

Western Blot Positive WB detected in: Rat Heart tissue, Rat Spleen tissue, Rat Brain tissue All lanes: PKM antibody at 1:4000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 55-60 kDa Observed band size: 55-60 kDa (PKM antibody (AA 2-531))

Western Blot Positive WB detected in: MCF-7 whole cell lysate at 40 μg, 20 μg, 10 μg, 5 μg, 2. (PKM antibody (AA 2-531))

Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5 min (PKM antibody (AA 2-531))

IHC

9 images

Catalog No. ABIN7166916

Datasheet as PDF

Target See all PKM Antibodies

PKM (Pyruvate Kinase M1/2 (PKM))
Binding Specificity
5

5

4

2

2

2

1

1

1

1

1

1
AA 2-531
Reactivity
36

11

5

1
Human
Host
34

4

1
Mouse
Compatible Secondaries
Clonality
34

5
Monoclonal
Conjugate
22

4

4

3

1

1

1

1

1

1
This PKM antibody is un-conjugated
Application
24

17

10

7

4

3

2

2

1

1
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Cross-Reactivity

Human, Mouse, Rabbit, Rat

Purification

Protein G purified

Immunogen

Recombinant Human Pyruvate kinase PKM protein (2-531AA)

Clone

6C3C7

Isotype

IgG1, IgG1 kappa
anti-Pyruvate Kinase M1/2 (PKM) antibody
PKM Reactivity: Human ELISA, WB, IHC, IF, IP, FACS Host: Rabbit Monoclonal 7B2 unconjugated Recombinant Antibody

anti-Pyruvate Kinase M1/2 (PKM) (AA 185-461) antibody
PKM Reactivity: Human ELISA, WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (Internal Region) antibody
PKM Reactivity: Human, Mouse, Rat ELISA, WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (AA 2-192) antibody
PKM Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibody
PKM Reactivity: Human, Mouse, Rat ELISA, WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibody
PKM Reactivity: Human ELISA, WB, IF Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) antibody
PKM Reactivity: Human, Mouse, Rat ELISA, WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (pTyr105) antibody
PKM Reactivity: Human, Mouse ELISA, WB, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (AA 475-500), (N-Term) antibody
PKM Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) antibody
PKM Reactivity: Human ELISA, WB, IP Host: Mouse Monoclonal unconjugated

Application Notes

Recommended dilution: WB: 1:4000-1:256000, IHC: 1:200-1:500, IF: 1:150-1:300, FC: 1:50-1:200, IP: 1 μL-4 μL,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name

PKM (PKM Products)

Synonyms

CTHBP antibody, OIP3 antibody, PK3 antibody, PKM2 antibody, TCB antibody, THBP1 antibody, AA414905 antibody, AL024370 antibody, AL024424 antibody, Pk-2 antibody, Pk-3 antibody, Pk3 antibody, Pkm2 antibody, PKM12 antibody, CG7070 antibody, Dmel\\CG7070 antibody, PYK antibody, Pyk antibody, cb1029 antibody, fj33f03 antibody, pkm2 antibody, pkm2a antibody, wu:fj33f03 antibody, MGC53388 antibody, PKM antibody, PK-S antibody, pk3 antibody, pkm2b antibody, zgc:92037 antibody, pyruvate kinase M1/2 antibody, pyruvate kinase, muscle antibody, Pyruvate kinase antibody, pyruvate kinase M1/2a antibody, pyruvate kinase PKM antibody, pyruvate kinase (PK) antibody, pyruvate kinase antibody, pyruvate kinase M1/2 S homeolog antibody, pyruvate kinase M1/2b antibody, PKM antibody, Pkm antibody, PyK antibody, pkma antibody, pkm antibody, LOC710798 antibody, pyk antibody, pk antibody, LOC100534543 antibody, pkm.S antibody, pkmb antibody

Background

Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Aliases: CTHBP antibody, Cytosolic thyroid hormone-binding protein antibody, KPYM_HUMAN antibody, OIP-3 antibody, Opa-interacting protein 3 antibody, p58 antibody, pkm antibody, PKM1 antibody, PKM2 antibody, Pyruvate kinase 2/3 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase PKM antibody, THBP1 antibody, Thyroid hormone-binding protein 1 antibody, Tumor M2-PK antibody

UniProt

P14618

Pathways

Warburg Effect