CPI-17 antibody (AA 30-126)
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- Target See all CPI-17 (PPP1R14A) Antibodies
- CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))
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Binding Specificity
- AA 30-126
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CPI-17 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Protein phosphatase 1 regulatory subunit 14A(PPP1R14A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Protein phosphatase 1 regulatory subunit 14A(PPP1R14A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: protein phosphatase 1 regulatory inhibitor subunit 14A
Protein Name: Protein phosphatase 1 regulatory subunit 14A - Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126). Human CPI17 alpha shares 83.5% and 84.5% amino acid (aa) sequence identity with mouse and rat CPI17 alpha, respectively.
- Isotype
- IgG
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))
- Alternative Name
- PPP1R14A (PPP1R14A Products)
- Synonyms
- MGC81874 antibody, cpi-17 antibody, ppp1inl antibody, CPI-17 antibody, im:7147597 antibody, ppp1r14a antibody, zgc:110700 antibody, PPP1R14A antibody, CPI17 antibody, PPP1INL antibody, Cpi17 antibody, 1110001M11Rik antibody, protein phosphatase 1 regulatory inhibitor subunit 14A L homeolog antibody, protein phosphatase 1 regulatory inhibitor subunit 14A antibody, protein phosphatase 1, regulatory (inhibitor) subunit 14Aa antibody, protein phosphatase 1, regulatory (inhibitor) subunit 14A antibody, ppp1r14a.L antibody, ppp1r14a antibody, ppp1r14aa antibody, PPP1R14A antibody, Ppp1r14a antibody
- Background
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Protein phosphatase 1 regulatory subunit 14A, also known as CPI-17, is a protein that in humans is encoded by the PPP1R14A gene. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Synonyms: 17-KDa protein | CPI 17 alpha | CPI 17 | CPI 17alpha | CPI-17 | CPI17 | PPP1INL | Ppp1r14a | Q96A00 - Gene ID
- 94274
- UniProt
- Q96A00
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