TNFSF4 Protein (Trimer) (His-DYKDDDDK Tag)
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- Target See all TNFSF4 Proteins
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- Protein Type
- Recombinant
- Protein Characteristics
- Trimer
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNFSF4 protein is labelled with His-DYKDDDDK Tag.
- Purpose
- Human OX40 Ligand/TNFSF4 Trimer Protein
- Sequence
- Gln51-Leu183
- Characteristics
- Recombinant Human OX40 Ligand/TNFSF4 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gln51-Leu183.
- Purity
- > 90 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human OX40 Ligand Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
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Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) protein (mFc-His Tag)
TNFSF4 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. FACS
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 51-183) (Active) protein (His tag)HPLC verified TNFSF4 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 51-198) protein (Fc Tag)TNFSF4 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 52-183) protein (His-DYKDDDDK-Strep II Tag)TNFSF4 Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) protein (His tag)TNFSF4 Origin: Cynomolgus Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 1-183) protein (GST tag)TNFSF4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 51-183) protein (His tag)Crystallography grade TNFSF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 51-198) protein (His tag)Crystallography grade TNFSF4 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 1-183) protein (Strep Tag)Crystallography grade TNFSF4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- Alternative Name
- OX40 Ligand (TNFSF4 Products)
- Synonyms
- TNFSF4 Protein, OX40L Protein, TNFRSF4 Protein, CD134L Protein, CD252 Protein, GP34 Protein, OX-40L Protein, OX4OL Protein, TXGP1 Protein, Ath-1 Protein, Ath1 Protein, Ox40l Protein, Txgp1l Protein, gp34 Protein, TNF superfamily member 4 Protein, tumor necrosis factor (ligand) superfamily, member 4 Protein, TNFSF4 Protein, Tnfsf4 Protein
- Background
- Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
- Molecular Weight
- 49.7 kDa. Due to glycosylation, the protein migrates to 65-140 kDa based on Tris-Bis PAGE result.
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints
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