For best experience we recommend to activate Javascript in your browser.

VCP antibody (N-Term)

Name: VCP antibody (N-Term)
Brand: antibodies-online
SKU: ABIN2856882
Price: 609.18 USD
Availability: InStock

VCP Reactivity: Human WB, IF, IP, IHC (p), ICC, IHC (wm) Host: Rabbit Polyclonal unconjugated

WB Image VCP antibody detects VCP protein by Western blot analysis. (VCP antibody (N-Term))

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97), antibody at 1:200 dilution. (VCP antibody (N-Term))

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7. (VCP antibody (N-Term))

IHC-P Image VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis. (VCP antibody (N-Term))

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97), antibody at 1:500 dilution. (VCP antibody (N-Term))

IHC

WB Image Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 , C: Molt-4 , D: Raji 7. (VCP antibody (N-Term))

PME-1 knockout reduces whole cell PP2A activity. (VCP antibody (N-Term))

PME-1 knockout reduces PP2A protein levels. (VCP antibody (N-Term))

8 images

(3 references)

Catalog No. ABIN2856882

Datasheet as PDF

Target See all VCP Antibodies

VCP (Valosin Containing Protein (VCP))
Binding Specificity
17

10

9

8

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reactivity
96

48

40

15

6

5

5

4

4

4

2

2

2

1

1

1

1
Human
Host
80

18

2
Rabbit
Compatible Secondaries
Clonality
71

29
Polyclonal
Conjugate
59

8

7

4

3

3

3

3

3

3

2

2
This VCP antibody is un-conjugated
Application
67

39

26

20

19

14

13

12

8

2

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity

Dog, Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics

Rabbit Polyclonal antibody to VCP (valosin-containing protein)
VCP antibody [N1N2], N-term

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

Isotype

IgG
anti-Valosin Containing Protein (VCP) (C-Term) antibody
KO Validated VCP Reactivity: Human WB, IF, IP, IHC (p), ICC, IHC (fro), IHC (wm) Host: Rabbit Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 125-371) antibody
VCP Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 1-588) antibody
VCP Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 4A8 unconjugated

anti-Valosin Containing Protein (VCP) (AA 647-806) antibody
VCP Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) antibody
VCP Reactivity: Human WB, ELISA, IHC, IF, IP Host: Rabbit Monoclonal 5H12 unconjugated Recombinant Antibody

anti-Valosin Containing Protein (VCP) antibody
VCP Reactivity: Human, Mouse WB, ICC, FACS Host: Mouse Monoclonal Hs-14 unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760), (C-Term) antibody
VCP Reactivity: Human, Mouse, Rat WB, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 749-766), (C-Term) antibody
VCP Reactivity: Human, Rat WB, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (C-Term) antibody
VCP Reactivity: Human, Mouse, Rat WB, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760) antibody
VCP Reactivity: Human, Mouse, Rat WB, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: Mouse brain , rat brain , A549 , H1299 , HCT116

Restrictions

For Research Use only
Format

Liquid

Concentration

0.3 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Tsuji, Ohama, Nakagawa, Sato: "Efficacy of an anti-cancer strategy targeting SET in canine osteosarcoma." in: The Journal of veterinary medical science, Vol. 81, Issue 10, pp. 1424-1430, (2020) (PubMed).

Kake, Tsuji, Enjoji, Hanasaki, Hayase, Yabe, Tanaka, Nakagawa, Liu, Chang, Usui, Ohama, Sato: "The role of SET/I2PP2A in canine mammary tumors." in: Scientific reports, Vol. 7, Issue 1, pp. 4279, (2019) (PubMed).

Yabe, Tsuji, Mochida, Ikehara, Usui, Ohama, Sato: "A stable association with PME-1 may be dispensable for PP2A demethylation - implications for the detection of PP2A methylation and immunoprecipitation." in: FEBS open bio, Vol. 8, Issue 9, pp. 1486-1496, (2018) (PubMed).
Target

VCP (Valosin Containing Protein (VCP))

Alternative Name

valosin containing protein (VCP Products)

Synonyms

ALS14 antibody, IBMPFD antibody, TERA antibody, p97 antibody, CDC48 antibody, wu:fd16d05 antibody, wu:fj63d11 antibody, p93 antibody, 3110001E05 antibody, p97/VCP antibody, 22.26 antibody, 7.12 antibody, Apple antibody, BcDNA.GM02885 antibody, BcDNA:GM02885 antibody, CG2331 antibody, DmTER94 antibody, Dmel\CG2331 antibody, I antibody, Ter94 antibody, Tera antibody, VCP antibody, VCP ATPase antibody, VCP/p97 antibody, Vcp antibody, anon-WO2004063362.65 antibody, anon-WO2004063362.67 antibody, cdc48 antibody, dTERA antibody, dVCP antibody, dmTERA antibody, dvcp antibody, l(2)03775 antibody, l(2)46CFf antibody, l(2)46CFs antibody, l(2)46Ch antibody, pTER94 antibody, ter94 antibody, vcp antibody, FANCG antibody, valosin containing protein antibody, valosin containing protein S homeolog antibody, valosin-containing protein antibody, CG2331 gene product from transcript CG2331-RA antibody, zgc:136908 antibody, transitional endoplasmic reticulum ATPase antibody, VCP antibody, vcp.S antibody, vcp antibody, Vcp antibody, Bm1_56585 antibody, LOC100136246 antibody, TER94 antibody, zgc:136908 antibody, LOC101109680 antibody

Molecular Weight

89 kDa

Gene ID

7415

UniProt

P55072

Pathways

ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway