HEK-293 Cells IgG2 Isotype Control

Details for Product No. ABIN2181275, Supplier: Log in to see
Isotype
  • FCGR2B
  • Fc fragment of IgG, low affinity IIb, receptor (CD32)
  • FCGR2B
Reactivity
Human
Host
HEK-293 Cells
9
7
6
6
6
6
6
5
5
3
2
2
2
1
Biological Activity
Active
Application
Isotype Control (IsoC)
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Isotype IgG2
Characteristics This protein carries no "tag". The protein has a calculated MW of 25.7 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind rhCD32a (Catalog # CDA-H5221) in a functional ELISA.
Background Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis. Ig gamma-2 chain Fc region contains two constant regions of IgG2 H chain (CH2, CH3).
Molecular Weight 25.7 kDa
UniProt P01859
Research Area Secondary Antibodies
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for HEK-293 Cells IgG2 Isotype Control (ABIN2181275) Human IgG2 Fc, Tag Free on SDS-PAGE under reducing (R) and no-reducing (NR) condition...
Background publications Sikorav, Auffray, Rougeon: "Structure of the constant and 3' untranslated regions of the murine Balb/c gamma 2a heavy chain messenger RNA." in: Nucleic acids research, Vol. 8, Issue 14, pp. 3143-55, 1981 (PubMed).

Ollo, Auffray, Morchamps, Rougeon: "Comparison of mouse immunoglobulin gamma 2a and gamma 2b chain genes suggests that exons can be exchanged between genes in a multigenic family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, Issue 4, pp. 2442-6, 1981 (PubMed).