Recombinant Human IgG2 Isotype Control
MALS verified
IsoC, ELISA, WB, IHC, NC, IF, in vivo, in vitro
Chimeric
IgG2
Recombinant Antibody
unconjugated
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- Target See all IgG2 products
- IgG2
- Antibody Type
- Recombinant Antibody
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Host
- Human
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Clonality
- Chimeric
- Application
- Isotype Control (IsoC), ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Negative Control (NC), Immunofluorescence (IF), In vivo Studies (in vivo), In vitro Assay (in vitro)
- Purpose
- Human IgG2 Kappa Isotype Control (mAb, carrier free, MALS verified)
- Specificity
- Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
- Characteristics
- Human IgG2 Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG2 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with DNP.
- Grade
- MALS verified
- Isotype
- IgG2
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HEK-293 Cells IgG2 isotype control
IsoC IgG2 unconjugated
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- Application Notes
- This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- -80 °C
- Storage Comment
- -70°C
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- Target
- IgG2
- Abstract
- IgG2 Products
- Synonyms
- FCGR2B Isotype Control, Fc fragment of IgG, low affinity IIb, receptor (CD32) Isotype Control, FCGR2B Isotype Control
- Target Type
- Antibody
- Background
- A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
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