Rabbit IgG Isotype Control

Details for Product No. ABIN398653, Supplier: Log in to see
Isotype
Host
Rabbit
218
210
208
172
162
130
114
94
93
92
91
76
59
52
44
42
27
25
24
23
22
11
8
6
3
3
1
Application
Blocking Reagent (BR), Dot Blot (DB), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Immunoprecipitation (IP), Isotype Control (IsoC), ELISA, Western Blotting (WB)
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Isotype IgG
Fragment Whole molecule
Characteristics Purified Rabbit IgG (Whole Molecule) Control is highly purified from normal rabbit serum by Protein G chromatography.Purified Rabbit IgG (Whole Molecule) Control is suitable for use as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunobloting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It also may be used as antigen or ligand in immunochemical reactions and conjugations.
Purification Protein G chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Each Investigator should determine their own optimal working dilution for specific applications.
Restrictions For Research Use only
Format Lyophilized
Concentration 4 mg/mL
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Product cited in: Timblin, Schlissel: "Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 9, pp. 4676-87, 2013 (PubMed).

Cowan, Singh, Ghert: "PTHrP increases RANKL expression by stromal cells from giant cell tumor of bone." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 30, Issue 6, pp. 877-84, 2012 (PubMed).

Cowan, Ghert, Singh: "T cells stimulate catabolic gene expression by the stromal cells from giant cell tumor of bone." in: Biochemical and biophysical research communications, Vol. 419, Issue 4, pp. 719-23, 2012 (PubMed).

Gee, Milgram, Kramer, Conlon, Moody: "Yes-associated protein 65 (YAP) expands neural progenitors and regulates Pax3 expression in the neural plate border zone." in: PLoS ONE, Vol. 6, Issue 6, pp. e20309, 2011 (PubMed).