Rat IgG Isotype Control
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- Target See all IgG products
- IgG
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Host
- Rat
- Application
- Isotype Control (IsoC), ELISA, Western Blotting (WB), Dot Blot (DB), Immunodiffusion (ID), Haemagglutination (H), Binding Studies (Bind)
- Characteristics
- Identity and purity is confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to rat total serum proteins, to rat IgG, IgA, IgM, transferrin and albumin
- Purification
- Chromatographically purified rat IgG
- Purity
- > = 96 % as determined by agar-gel electrophoresis. Tested in a concentration of 70.0 mg/mL at the level of sensitivity of the applied immunoprecipitating techniques, this product contains only traces of IgA, IgM, transferrin and albumin.
- Isotype
- IgG
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Rabbit IgG isotype control
IsoC, ELISA, WB IgG Native unconjugated
Mouse IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlELISA, WB, IHC IgG unconjugated
Rabbit IgG isotype control (PE)ELISA, FACS Polyclonal IgG PE
Goat IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Rabbit IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rat IgG isotype controlELISA, WB IgG unconjugated
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- Application Notes
- Purified rat IgG may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelec-trophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
- Comment
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Lyophilized, stable white powder.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- After opening the vial, the lyophilized content is reconstituted by adding 1 mL of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium.
- Preservative
- Without preservative
- Handling Advice
- Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
- Storage
- 4 °C/-20 °C
- Storage Comment
- This product is shipped lyophilized at ambient temperature. After arrival store at +4 °C. Prolonged storage may be at -20 °C. Reconstituted solution may be stored frozen.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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