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anti-Mouse (Murine) Angiotensin II Type-1 Receptor antibody for ELISA

Recommended Angiotensin II Type-1 Receptor Antibody (supplied by: Log in to see )

Antigen
Angiotensin II Receptor, Type 1 (AGTR1) Antibodies
  • 1810074K20Rik
  • AG2S
  • Agtr-1a
  • Agtr-1b
  • Agtr1
  • AGTR1
  • agtr1-A
  • agtr1.2
  • AGTR1A
  • AGTR1B
  • AGTR2
  • AI551199
  • Angtr-1a
  • Angtr-1b
  • AT1
  • at1
  • AT1-R
  • AT1a
  • AT1AR
  • AT1B
  • AT1BR
  • AT1R
  • at2
  • at2r
  • AT2R1
  • AT2R1A
  • AT2R1B
  • AT3
  • AT1R
  • atgr2
  • HAT1R
  • mrx88
  • XAT-1
  • zgc:113016
Reactivity
Human, Mouse (Murine), Rat (Rattus)
78
55
47
5
2
2
2
1
1
1
Host
Goat
66
15
6
5
4
Clonality
Polyclonal
Conjugate
This Angiotensin II Type-1 Receptor antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
79
53
21
14
12
11
6
4
2
1
1
Supplier
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Catalog No. ABIN188713
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012045928 ABIN190902 ELISA WB Goat Internal Region Log in to see Polyclonal 1
0.0012045928 ABIN1582292 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal 2
0.0012045928 ABIN1535649 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012045928 ABIN2465366 ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012045928 ABIN2458631 ELISA WB Rabbit Log in to see Polyclonal
0.0012045928 ABIN4278728 ELISA ICC IF IHC IHC (p) WB Rabbit Center Log in to see Polyclonal 4
0.0012045928 ABIN2162465 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012045928 ABIN1731610 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012045928 ABIN1454244 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012045928 ABIN1030844 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.0012045928 ABIN2421147 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012045928 ABIN2427725 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012045928 ABIN2155087 IHC ELISA WB Rabbit Log in to see Polyclonal
0.0012045928 ABIN342802 IP ELISA WB Sheep AA 297-359 Log in to see Polyclonal
0.0012045928 ABIN2560976 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.0012045928 ABIN2861333 IF IHC ELISA WB Rabbit Log in to see Polyclonal
0.0012045928 ABIN4278727 ELISA ICC IF IHC IHC (p) WB Rabbit Center Log in to see Polyclonal
0.0012045928 ABIN1887578 IHC ELISA WB Rabbit Center Log in to see Polyclonal

Top referenced anti-Angiotensin II Type-1 Receptor antibody for ELISA

Similar anti-Angiotensin II Type-1 Receptor Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 32 Antibodies 19 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 3 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 5 Antibodies
Immunofluorescence (IF) 7 Antibodies 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 9 Antibodies
Western Blotting (WB) 42 Antibodies 37 Antibodies
Immunoprecipitation (IP) 1 Antibodies

General

Antigen Angiotensin II Receptor, Type 1 (AGTR1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(78), (55), (47), (5), (2), (2), (2), (1), (1), (1)
Host Goat
(66), (15), (6), (5), (4)
Clonality
Polyclonal
Conjugate This Angiotensin II Type-1 Receptor antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(79), (53), (21), (14), (12), (11), (6), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-Angiotensin II Type-1 Receptor Antibody

Target Details Angiotensin II Type-1 Receptor Application Details Handling References for anti-Angiotensin II Type-1 Receptor Antibody (ABIN188713) Images
Specificity All reported variants represent identical protein (NP_000676.1, NP_004826.2, NP_033611.1, NP_114038.1 and NP_114438.1).
Purification Immunogen affinity purified
Immunogen Peptide with sequence C-EIQKNKPRNDDIFK corresponding to internal region according to NP_004826.2, NP_033611.1, NP_114038.1, NP_114438.1.
Isotype IgG

Target Details Angiotensin II Type-1 Receptor

Product Details anti-Angiotensin II Type-1 Receptor Antibody Application Details Handling References for anti-Angiotensin II Type-1 Receptor Antibody (ABIN188713) Images back to top
Antigen
Alternative Name AGTR-1 (AGTR1 Antibody Abstract)
Background Gene Symbol: AGTR1
Molecular Weight Theoretical MW: 41 kDa
Gene ID 185, 11607, 24180
UniProt P30556
Pathways JAK-STAT Signaling, ACE Inhibitor Pathway

Application Details

Product Details anti-Angiotensin II Type-1 Receptor Antibody Target Details Angiotensin II Type-1 Receptor Handling References for anti-Angiotensin II Type-1 Receptor Antibody (ABIN188713) Images back to top
Application Notes Western Blot 0.3 - 1 μg/mL, Immunohistochemistry 1:10 - 1:500, Peptide ELISA Detection limit 1:32000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Angiotensin II Type 1 Receptor Antibody TISSUE LYSIS:Tissue chunks were weighed and cut into approx 1mm cubes using a razor blade. The tissue was transferred to a handheld homogenizer and 3 mL of ice-cold RIPA buffer was added per 1g of tissue. The tissue was gently homogenised over 20 minutes on ice. The resulting lysate was aliquotted into 1.5 mL microfuge tubes and centrifuged at 13,000 rpm for 5 min in a microfuge. The supernatant was transferred into clean tubes and its protein concentration was measured with BioRad protein assay. The concentration was then adjusted to 5 mg/ml with RIPA lysis buffer. An equal volume of 2 x SDS sample buffer was added and the cell lysate was boiled for 5 minutes. Lysates were stored at -80C until use.(RIPA buffer = 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 µg/ml Aprotinin, 5 µg/ml Leupeptin, 1 % Triton X-100, 1 % Sodium deoxycholate, 0.1 % SDS).SDS PAGE:Samples were run at 200V constant on a 12 % acrylamide SDS-PAGE mini gel - using Biorad Mini-Protean 3 kit and protocols. Before loading samples had 5 % (v/v) 2-ME added and were boiled for 3 minutes.TRANSFER:We used a Biorad Mini Trans-Blot, constant 100 V for 1 hour. Transfer
Restrictions For Research Use only

Handling

Product Details anti-Angiotensin II Type-1 Receptor Antibody Target Details Angiotensin II Type-1 Receptor Application Details References for anti-Angiotensin II Type-1 Receptor Antibody (ABIN188713) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 with 0.5 % BSA.
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-Angiotensin II Type-1 Receptor Antibody (ABIN188713)

Product Details anti-Angiotensin II Type-1 Receptor Antibody Target Details Angiotensin II Type-1 Receptor Application Details Handling Images back to top
Product cited in:

Marut, Kavian, Servettaz, Hua-Huy, Nicco, Chéreau, Weill, Dinh-Xuan, Batteux: "Amelioration of systemic fibrosis in mice by angiotensin II receptor blockade." in: Arthritis and rheumatism, Vol. 65, Issue 5, pp. 1367-77, 2013 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Wang, Jiang, Wang, Yao: "Role of brain angiotensin AT1 receptor in the carbachol-induced natriuresis and expression of nNOS in the locus coeruleus and proximal convoluted tubule." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 56, Issue 4, pp. 383-91, 2007

Images

Product Details anti-Angiotensin II Type-1 Receptor Antibody Target Details Angiotensin II Type-1 Receptor Application Details Handling References for anti-Angiotensin II Type-1 Receptor Antibody (ABIN188713) back to top
Supplier Images
Western Blotting (WB) image for anti-Angiotensin II Type-1 Receptor antibody (Angiotensin II Receptor, Type 1) (ABIN188713) Western Blot: AGTR1 Antibody [ABIN188713] - ABIN188713 (0.3ug/ml) staining of Mouse L...