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anti-Mouse (Murine) CD25 antibody for Blocking Reagent

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Mouse (Murine)
577
461
170
19
10
10
8
5
4
3
3
2
1
1
Host
Rat
609
410
135
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
125
102
61
30
28
25
25
22
19
18
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS)
947
149
142
132
130
125
84
74
48
21
20
18
17
11
10
9
6
5
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN1176951
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN2689118 BR FACS IP IHC Rat IgG1, lambda Log in to see PC61 13
0.0008709465 ABIN1176950 BR FACS ICFC Rat IgG2b kappa Log in to see 3C7
0.0008709465 ABIN1967959 BR FACS Mouse IgG1 Log in to see NYRmIL-2R

Top referenced anti-CD25 antibody for Blocking Reagent

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 15 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 403 Antibodies
Functional Studies (Func) 28 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 51 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 90 Antibodies
In vitro Assay (in vitro) 4 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(577), (461), (170), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Rat
(609), (410), (135), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (125), (102), (61), (30), (28), (25), (25), (22), (19), (18), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS)
(947), (149), (142), (132), (130), (125), (84), (74), (48), (21), (20), (18), (17), (11), (10), (9), (6), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1176951) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen IL-2-dependent cytolytic mouse T-cell clone B6.1
Clone PC61
Isotype IgG1, lambda

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1176951) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor alpha chain (IL-2Ralpha, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Ralpha by itself is not a signaling receptor. However, it can combine with IL-2 Receptor beta (CD122) and gammac (CD132) chains to c form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Ralpha. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat. No. 557364) and 7D4 (Cat. No. 553068). It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25. Use of this product can fall under one or more claims of the following patents licensed to Becton, Dickinson and Company: US Patent Nos. 5,445,939, 5,656,446, 5,843,689, European Patent No. 319,543, Canadian Patent No. 1,296,622, Australian Patent No. 615,880, and Japanese Patent No. 2,769,156.
Synonyms: Interleukin-2 receptor alpha chain, IL-2RA, IL-2Ralpha, Il2ra, IL-2R p55
Research Area Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1176951) Images back to top
Application Notes Flow Cytometry: Investigators may also wish to consider using PE Rat Anti-Mouse CD25 (Cat. No. 553866) or Biotin Rat Anti-Mouse CD25 (Cat. No. 553069) coupled with PE Streptavidin (Cat. No. 554061) for samples expressing low levels of CD25.
Immunohistochemistry: Investigators are encouraged to consider using Biotin Rat Anti-Mouse CD25 (Cat. No. 550529) for the immunohistochemistry application.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1176951) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD25 Antibody (ABIN1176951)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, 2000

Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi, Mak, Sakaguchi: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, 2000

Chen, Ma, Young, Alt: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, 1995

Rolink, Grawunder, Winkler, Karasuyama, Melchers: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." in: International immunology, Vol. 6, Issue 8, pp. 1257-64, 1995

Godfrey, Zlotnik: "Control points in early T-cell development." in: Immunology today, Vol. 14, Issue 11, pp. 547-53, 1994

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Garni-Wagner, Witte, Tutt, Kuziel, Tucker, Bennett, Kumar: "Natural killer cells in the thymus. Studies in mice with severe combined immune deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 796-803, 1990

Pollard, Lipscomb: "Characterization of murine lung dendritic cells: similarities to Langerhans cells and thymic dendritic cells." in: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 159-67, 1990

Crowley, Inaba, Witmer-Pack, Steinman: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, 1989

Moreau, Nabholz, Diamantstein, Malek, Shevach, Thèze: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987

Ceredig, Lowenthal, Nabholz, MacDonald: "Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells." in: Nature, Vol. 314, Issue 6006, pp. 98-100, 1985

Lowenthal, Corthésy, Tougne, Lees, MacDonald, Nabholz: "High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 6, pp. 3988-94, 1985

Lowenthal, Zubler, Nabholz, MacDonald: "Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." in: Nature, Vol. 315, Issue 6021, pp. 669-72, 1985