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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||16 Antibodies|
|Blocking Reagent (BR)||4 Antibodies|
|Cytotoxicity Test (CyTox)||1 Antibodies|
|ELISA (Detection)||3 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Flow Cytometry (FACS)||280 Antibodies|
|Functional Studies (Func)||25 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||34 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||23 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||90 Antibodies|
|In vitro Assay (in vitro)||4 Antibodies|
|Intracellular Flow Cytometry (ICFC)||17 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD25 antibody is un-conjugated Alternatives|
Blocking Reagent (BR), Flow Cytometry (FACS)
|13 references available|
|Supplier||Log in to see|
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1176951) Images
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.|
|Immunogen||IL-2-dependent cytolytic mouse T-cell clone B6.1|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1176951) Images back to top
|Alternative Name||CD25 (IL2RA Antibody Abstract)|
The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor alpha chain (IL-2Ralpha, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Ralpha by itself is not a signaling receptor. However, it can combine with IL-2 Receptor beta (CD122) and gammac (CD132) chains to c form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Ralpha. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat. No. 557364) and 7D4 (Cat. No. 553068). It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25. Use of this product can fall under one or more claims of the following patents licensed to Becton, Dickinson and Company: US Patent Nos. 5,445,939, 5,656,446, 5,843,689, European Patent No. 319,543, Canadian Patent No. 1,296,622, Australian Patent No. 615,880, and Japanese Patent No. 2,769,156.
Synonyms: Interleukin-2 receptor alpha chain, IL-2RA, IL-2Ralpha, Il2ra, IL-2R p55
|Research Area||Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1176951) Images back to top
Flow Cytometry: Investigators may also wish to consider using PE Rat Anti-Mouse CD25 (Cat. No. 553866) or Biotin Rat Anti-Mouse CD25 (Cat. No. 553069) coupled with PE Streptavidin (Cat. No. 554061) for samples expressing low levels of CD25.
Immunohistochemistry: Investigators are encouraged to consider using Biotin Rat Anti-Mouse CD25 (Cat. No. 550529) for the immunohistochemistry application.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1176951) Images back to top
|Buffer||No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.|
|Storage Comment||Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.|
References for anti-CD25 Antibody (ABIN1176951)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
|Product cited in:||
Takahashi, Tagami, Yamazaki et al.: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, 2000 (PubMed).
Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, 2000 (PubMed).
Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993 (PubMed).
Chen, Ma, Young et al.: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, 1995 (PubMed).
Godfrey, Zlotnik: "Control points in early T-cell development." in: Immunology today, Vol. 14, Issue 11, pp. 547-53, 1994 (PubMed).
Rolink, Grawunder, Winkler et al.: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." in: International immunology, Vol. 6, Issue 8, pp. 1257-64, 1995 (PubMed).
Lowenthal, Corthésy, Tougne et al.: "High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 6, pp. 3988-94, 1985 (PubMed).
Lowenthal, Zubler, Nabholz et al.: "Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." in: Nature, Vol. 315, Issue 6021, pp. 669-72, 1985 (PubMed).
Ceredig, Lowenthal, Nabholz et al.: "Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells." in: Nature, Vol. 314, Issue 6006, pp. 98-100, 1985 (PubMed).
Crowley, Inaba, Witmer-Pack et al.: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, 1989 (PubMed).
Moreau, Nabholz, Diamantstein et al.: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987 (PubMed).
Pollard, Lipscomb: "Characterization of murine lung dendritic cells: similarities to Langerhans cells and thymic dendritic cells." in: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 159-67, 1990 (PubMed).
Garni-Wagner, Witte, Tutt et al.: "Natural killer cells in the thymus. Studies in mice with severe combined immune deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 796-803, 1990 (PubMed).