CD25 antibody (Interleukin 2 Receptor, alpha) (Biotin)

Details for Product anti-IL2RA Antibody No. ABIN94045
Antigen
Reactivity
Human
470
299
101
17
15
6
6
5
5
4
4
3
1
Host
Mouse
414
240
154
4
3
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD25 antibody is conjugated to Biotin
108
105
79
62
16
11
10
10
9
9
7
5
5
5
5
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
616
140
120
113
95
89
49
39
26
17
15
14
13
12
8
4
2
2
2
2
1
1
1
1
1
1
1
1
1
Options
Immunogen PHA-activated peripheral blood leucocytes
Clone MEM-181
Isotype IgG1
Specificity The antibody MEM-181 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells), it is lost on resting B and T lymphocytes.
HLDA VI, WS Code NL N-L024
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 Molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
Application Notes The reagent is designed for Flow Cytometry analysis.Suggested working dilution is 1:500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (Biotin) (ABIN94045) Surface staining of human PBMC with anti-human CD25 (MEM-181) FITC. The mononuclear c...
Product cited in: Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).