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anti-Mouse (Murine) CD25 antibody for Immunocytochemistry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Epitope
AA 34-139
22
20
19
18
15
15
14
7
7
6
6
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Monkey, Rat (Rattus)
560
456
155
19
15
9
7
4
4
3
3
2
1
1
Host
Mouse
565
399
137
7
5
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
133
123
95
57
28
26
25
24
22
20
17
14
12
11
11
11
11
11
10
10
8
8
8
6
6
5
5
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
908
154
152
148
113
84
78
71
45
21
19
17
15
14
13
12
10
9
7
6
6
5
4
3
3
2
2
1
1
1
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Catalog No. ABIN1724861
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN153451 ICC IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see IL2R-1 5
1 ABIN4880789 ELISA ICC IHC WB Mouse IgG1 AA 34-139 Log in to see 1B5D12
1 ABIN4291000 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 1B5D12
1 ABIN1859413 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-235 Log in to see Polyclonal
1 ABIN1823504 ICC IHC (p) ELISA WB Mouse IgG1 AA 34-139 Log in to see 1B5D12
1 ABIN4290998 ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see IL2R-1

Top referenced anti-CD25 antibody for Immunocytochemistry

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Application / Reactivity Rat (Rattus)
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Blocking Reagent (BR) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
ELISA 47 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 112 Antibodies
Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 21 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 46 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 34-139
(22), (20), (19), (18), (15), (15), (14), (7), (7), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Monkey, Rat (Rattus)
(560), (456), (155), (19), (15), (9), (7), (4), (4), (3), (3), (2), (1), (1)
Host Mouse
(565), (399), (137), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(133), (123), (95), (57), (28), (26), (25), (24), (22), (20), (17), (14), (12), (11), (11), (11), (11), (11), (10), (10), (8), (8), (8), (6), (6), (5), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(908), (154), (152), (148), (113), (84), (78), (71), (45), (21), (19), (17), (15), (14), (13), (12), (10), (9), (7), (6), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1724861) Images
Purification Purified
Immunogen Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.
Clone 1B5D12
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1724861) Images back to top
Antigen
Alternative Name IL2RA (IL2RA Antibody Abstract)
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. , , , ,
Molecular Weight 30.8 kDa
Gene ID 3559
HGNC 3559
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1724861) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1724861) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD25 Antibody (ABIN1724861)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, 2012

Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, 2012

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1724861) back to top
Supplier Images
ELISA image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
Immunocytochemistry (ICC) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 2)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 3)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 4)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 5)