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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||16 Antibodies|
|Blocking Reagent (BR)||4 Antibodies|
|Cytotoxicity Test (CyTox)||1 Antibodies|
|ELISA (Detection)||3 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Flow Cytometry (FACS)||280 Antibodies|
|Functional Studies (Func)||25 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||34 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||23 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||90 Antibodies|
|In vitro Assay (in vitro)||4 Antibodies|
|Intracellular Flow Cytometry (ICFC)||17 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD25 antibody is conjugated to Biotin Alternatives|
Flow Cytometry (FACS), Immunoprecipitation (IP)
|2 references available|
|Supplier||Log in to see|
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN135173) Images
The antibody detects: Low affinity α chain IL-2 receptor (IL-2Rα), Mr 55 kDa.
The monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody. While the monoclonal antibody does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antobody it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.
To insure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to charactersistics of a standard reference reagent.
|Immunogen||IL-2-dependent BALB/c mouse helper T-cell clone HT-2|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN135173) Images back to top
|Alternative Name||CD25/IL-2Ralpha (IL2RA Antibody Abstract)|
|Background||The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN135173) Images back to top
|Application Notes||Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.|
|Sample Volume||1 mL|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN135173) Images back to top
|Buffer||PBS / NaN3|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
References for anti-CD25 Antibody (ABIN135173)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Malek, Robb, Shevach: "Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 18, pp. 5694-8, 1983 (PubMed).
Lorenzo, Jaulin, Vita et al.: "Structure-function study of the p55 subunit of murine IL-2 receptor by epitope mapping." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 9, pp. 2970-7, 1991 (PubMed).
ImagesProduct Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN135173) back to top