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anti-Rabbit CD25 antibody for Immunoprecipitation

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Rabbit
443
332
109
19
10
10
8
5
3
3
3
2
1
Host
Mouse
481
303
89
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
114
113
91
61
28
17
15
13
12
10
10
7
6
6
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP)
675
129
117
95
92
70
59
47
45
21
17
15
14
11
10
9
6
5
5
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN2689120
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN2479464 IHC (fro) FACS IP Mouse IgG2b Log in to see KEI-alpha1 2
0.00089235755 ABIN2224484 FACS IP IHC Mouse IgG2b Log in to see
0.00089235755 ABIN2288562 FACS IP IHC Mouse IgG2b Log in to see 10B1126

Top referenced anti-CD25 antibody for Immunoprecipitation

Similar anti-CD25 Antibodies

Application / Reactivity Rabbit
Blocking Reagent (BR) 1 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunoprecipitation (IP) 4 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Rabbit
(443), (332), (109), (19), (10), (10), (8), (5), (3), (3), (3), (2), (1)
Host Mouse
(481), (303), (89), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(114), (113), (91), (61), (28), (17), (15), (13), (12), (10), (10), (7), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP)
(675), (129), (117), (95), (92), (70), (59), (47), (45), (21), (17), (15), (14), (11), (10), (9), (6), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2689120) Images
Brand BD Pharmingen™
Characteristics The Kei-α1 antibody reacts with the rabbit homolog of CD25, the interleukin-2 receptor alpha chain (IL-2Rα). mAb Kei-α1 recognizes specifically IL-2Rα and neither the IL-2R ß chain (CD122) nor the 25- kDa protein component of the rabbit high-affinity IL-2R complex. Based on flow cytometric analysis, fewer than 1 % of resting peripheral blood T and B cells have been found to stain with Kei-α1, while a majority of PHA blasts show intense staining with Kei-α1. The HTLV-1-transformed rabbit cell lines, F648b and YR-1 both also show intense staining with Kei-α1. mAb Kei-α1 has been reported to block both high-and low-affinity IL-2 binding and also cell proliferation of rabbit PHA blasts induced by picomolar concentrations of IL-2.

BD Pharmingen™ Purified Mouse Anti-Rabbit CD25 - Purified - Clone Kei-α1 - Isotype Mouse IgG2b, κ - Reactivity Rab - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Membrane preparation of the HTLV-1-transformed rabbit T-cell line F648b
Clone Kei
Isotype IgG2b, kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2689120) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background Synonyms: IL-2R alpha
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2689120) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2689120) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD25 Antibody (ABIN2689120)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Kotani, Yamamura, Tsudo, Tamatani, Kitamura, Miyasaka: "Generation of monoclonal antibodies to the rabbit interleukin-2 receptor alpha chain (CD25) and its distribution in HTLV-1-transformed rabbit T cells." in: Japanese journal of cancer research : Gann, Vol. 84, Issue 7, pp. 770-5, 1993