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anti-Rat (Rattus) CD25 antibody for Blocking Reagent

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Rat (Rattus)
569
457
168
19
10
10
8
5
4
3
3
2
1
1
Host
Mouse
601
406
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
130
124
101
61
30
28
25
25
22
19
17
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
936
144
141
129
127
120
80
72
48
21
18
18
17
11
10
9
6
5
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN2689119
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Top referenced anti-CD25 antibody for Blocking Reagent

Similar anti-CD25 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 1 Antibodies
ELISA 65 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 136 Antibodies
Functional Studies (Func) 7 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 60 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 30 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 41 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Rat (Rattus)
(569), (457), (168), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(601), (406), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(130), (124), (101), (61), (30), (28), (25), (25), (22), (19), (17), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(936), (144), (141), (129), (127), (120), (80), (72), (48), (21), (18), (18), (17), (11), (10), (9), (6), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2689119) Images
Brand BD Pharmingen™
Characteristics The OX-39 antibody reacts with the α chain of the IL-2 receptor on T lymphoblasts and thymic and splenic dendritic cells. CD25 has also been detected on rat intestinal epithelial cells. It has been reported that OX-39 mAb weakly blocks binding of IL-2 to T-cell blasts and that it blocks IL-2 stimulated epithelial cell migration in an in vitro model of wound healing. Use of this product can fall under one or more claims of the following patents licensed to Becton, Dickinson and Company: US Patent Nos. 5,445,939, 5,656,446, 5,843,689, European Patent No. 319,543, Canadian Patent No. 1,296,622, Australian Patent No. 615,880, and Japanese Patent No. 2,769,156. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Rat CD25 - Purified - Clone OX-39 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat T blasts from mixed-lymphocyte reactions
Clone OX
Isotype IgG1, kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2689119) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background Synonyms: IL-2R α Chain
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2689119) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2689119) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD25 Antibody (ABIN2689119)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Josien, Heslan, Soulillou, Cuturi: "Rat spleen dendritic cells express natural killer cell receptor protein 1 (NKR-P1) and have cytotoxic activity to select targets via a Ca2+-dependent mechanism." in: The Journal of experimental medicine, Vol. 186, Issue 3, pp. 467-72, 1997

Dignass, Podolsky: "Interleukin 2 modulates intestinal epithelial cell function in vitro." in: Experimental cell research, Vol. 225, Issue 2, pp. 422-9, 1996

Paterson, Jefferies, Green, Brandon, Corthesy, Puklavec, Williams: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, 1988

Tellides, Dallman, Kupiec-Weglinski, Diamantstein, Morris: "Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy." in: Transplantation proceedings, Vol. 19, Issue 5, pp. 4231-3, 1987