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anti-Rat (Rattus) CD25 antibody for Western Blotting

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Epitope
AA 34-139
22
20
14
13
12
11
7
7
7
6
5
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Monkey, Rat (Rattus)
568
456
168
19
10
10
8
4
4
3
3
2
1
1
Host
Mouse
601
406
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
130
124
101
61
30
28
25
25
22
19
17
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
937
145
140
128
126
121
80
72
48
21
18
17
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN1724861
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.125708 ABIN2705770 WB Rabbit Center Log in to see Polyclonal
0.125708 ABIN1873210 WB Rabbit IgG Log in to see Polyclonal
0.125708 ABIN1175093 IHC ELISA WB Biotin Rabbit IgG AA 24-225 Log in to see Polyclonal
0.125708 ABIN1859414 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 23-235 Log in to see Polyclonal
0.125708 ABIN1175091 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 23-235 Log in to see Polyclonal
0.125708 ABIN2405718 WB Rabbit IgG Log in to see Polyclonal
0.125708 ABIN1845343 ICC IF IHC ELISA WB Mouse IgG1 AA 34-139 Log in to see
0.125708 ABIN2426093 ELISA WB Rabbit IgG Log in to see Polyclonal
0.001176447 ABIN254758 FACS WB FITC Mouse IgG1 Log in to see OX-39
0.001176447 ABIN670371 IF (p) IHC (p) WB Rabbit IgG AA 258-268 Log in to see Polyclonal
0.001176447 ABIN1584197 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.001176447 ABIN603356 WB Biotin Sheep IgG Extracellular Domain Log in to see Polyclonal
0.001176447 ABIN670373 IHC (p) WB Biotin Rabbit IgG AA 258-268 Log in to see Polyclonal
0.001176447 ABIN670380 IHC (p) WB HRP Rabbit IgG AA 258-268 Log in to see Polyclonal
0.001176447 ABIN4291105 FACS WB Alexa Fluor 405 Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
0.001176447 ABIN4291106 FACS WB Alexa Fluor 488 Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
0.001176447 ABIN4291107 FACS WB Alexa Fluor 647 Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
0.001176447 ABIN4291108 FACS WB Alexa Fluor 700 Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
0.001176447 ABIN4291109 FACS WB Allophycocyanin Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
0.001176447 ABIN4291110 FACS WB Biotin Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab

Top referenced anti-CD25 antibody for Western Blotting

  • Human Monoclonal CD25 Primary Antibody for ICC, IHC - ABIN1724861 (2 Publications): Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients. in Annals of hematology 2012 (PubMed)

Similar anti-CD25 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 1 Antibodies 4 Antibodies
ELISA 65 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 136 Antibodies
Functional Studies (Func) 7 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 60 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 30 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 41 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 34-139
(22), (20), (14), (13), (12), (11), (7), (7), (7), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Monkey, Rat (Rattus)
(568), (456), (168), (19), (10), (10), (8), (4), (4), (3), (3), (2), (1), (1)
Host Mouse
(601), (406), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(130), (124), (101), (61), (30), (28), (25), (25), (22), (19), (17), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(937), (145), (140), (128), (126), (121), (80), (72), (48), (21), (18), (17), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1724861) Images
Purification Purified
Immunogen Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.
Clone 1B5D12
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1724861) Images back to top
Antigen
Alternative Name IL2RA (IL2RA Antibody Abstract)
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. , , , ,
Molecular Weight 30.8 kDa
Gene ID 3559
HGNC 3559
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1724861) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1724861) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD25 Antibody (ABIN1724861)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, 2012

Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, 2012

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1724861) back to top
Supplier Images
ELISA image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
Immunocytochemistry (ICC) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 2)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 3)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 4)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 5)