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Human CD25 ELISA Kit

Recommended CD25 Product (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) ELISA Kits
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Epitope
AA 22-213
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
37
13
9
5
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
78-5000 pg/mL
Minimum Detection Limit
78 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672737
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32748017 ABIN2472085 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.32748017 ABIN417245 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32748017 ABIN924856 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.32748017 ABIN1115648 Sandwich ELISA Serum, Plasma, Biological Fluids 93.75-6000 pg/mL 93.75 pg/mL Log in to see
0.32748017 ABIN1029824 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.32748017 ABIN2708136 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see
0.32748017 ABIN2708137 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.32748017 ABIN2115192 Cell Culture Supernatant, Serum, Plasma Log in to see
0.0012092679 ABIN365801 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012092679 ABIN1445998 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples Log in to see 3
0.0012092679 ABIN626507 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see
0.0012092679 ABIN455616 Serum, Plasma, Biological Fluids 78-5000 pg/mL 78 pg/mL Log in to see
0.0012092679 ABIN2684679 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 10-2000 pg/mL 10 pg/mL Log in to see 4
0.0012092679 ABIN1381233 Cell ELISA Log in to see
0.0012092679 ABIN625322 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 15-4000 pg/mL 15 pg/mL Log in to see
0.0012092679 ABIN2748780 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.3-2000 pg/mL 31.3 pg/mL Log in to see
0.0012092679 ABIN2042395 Sandwich ELISA 8-5000 pg/mL 8 pg/mL Log in to see
0.0012092679 ABIN2114292 Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.0012092679 ABIN2684680 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 78-5000 pg/mL 78 pg/mL Log in to see
0.0012092679 ABIN1446405 Cell Culture Supernatant, Plasma, Serum Log in to see

Top referenced CD25 ELISA Kit for ELISA

Similar CD25 ELISA Kits

Application / Reactivity Human
ELISA 37 ELISA Kits

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) ELISA Kits
Epitope AA 22-213
Reactivity Human
Kits with alternative reactivity to:
(37), (13), (9), (5), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 78-5000 pg/mL
Minimum Detection Limit 78 pg/mL
Application ELISA
Pubmed 2 references available
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Product Details CD25 ELISA Kit

Target details Application Details Handling References for CD25 Kit (ABIN1672737) Images
Purpose For quantitative detection of human CD25 in cell culture supernates, serum and plasma( heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human CD25
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <5pg/mL
Components
  • 96-well plate precoated with anti- human CD25 antibod - 1
  • Lyophilized recombinant human CD25 standar - 10ng/tubex2
  • Biotinylated anti- human CD25 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: E22-C213

Target details

Product Details CD25 ELISA Kit Application Details Handling References for CD25 Kit (ABIN1672737) Images back to top
Antigen
Alternative Name IL2RA (IL2RA ELISA Kit Abstract)
Background CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
Gene ID 3559
UniProt P01589
Pathways JAK-STAT Signaling

Application Details

Product Details CD25 ELISA Kit Target details Handling References for CD25 Kit (ABIN1672737) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human CD25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD25 has been precoated onto 96-well plates. Standards (NSO,E22-C213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD25 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human CD25 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CD25 standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 0.22, Standard deviation: 0.014, CV(%): 6.3
  • Sample 2: n=16, Mean(ng/ml): 0.63, Standard deviation: 0.041, CV(%): 6.5
  • Sample 3: n=16, Mean(ng/ml): 2.53, Standard deviation: 0.132, CV(%): 5.2,
  • Sample 1: n=24, Mean(ng/ml): 0.25, Standard deviation: 0.020, CV(%): 8
  • Sample 2: n=24, Mean(ng/ml): 0.71, Standard deviation: 0.052, CV(%): 7.3
  • Sample 3: n=24, Mean(ng/ml): 2.36, Standard deviation: 0.191, CV(%): 8.1
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CD25 Kit (ABIN1672737)

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Background publications

Ferrari, Cannizzaro, Battini, Huebner, Baserga: "The gene encoding human vimentin is located on the short arm of chromosome 10." in: American journal of human genetics, Vol. 41, Issue 4, pp. 616-26, 1987

Leonard, Depper, Kanehisa, Krönke, Peffer, Svetlik, Sullivan, Greene: "Structure of the human interleukin-2 receptor gene." in: Science (New York, N.Y.), Vol. 230, Issue 4726, pp. 633-9, 1985

Images

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Supplier Images
ELISA image for CD25 ELISA Kit (Interleukin 2 Receptor, alpha) (ABIN1672737) Human CD25/IL-2sR alpha PicoKine ELISA Kit standard curve