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anti-Human CSF2RB antibody for Western Blotting

Recommended CSF2RB Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage) (CSF2RB) Antibodies
  • AI848964
  • AIC2B
  • Bc
  • CD131
  • cd131
  • CDw131
  • cdw131
  • CSF2RB
  • Csf2rb1
  • Csfgmrb
  • il-3rbc
  • Il3r
  • Il3rb
  • IL3RB
  • il3rb
  • Il3rb1
  • Il5rb
  • il5rb
  • IL5RB
  • SMDP5
Epitope
C-Term
14
11
11
9
6
6
5
5
2
1
1
1
1
1
1
Reactivity
Human
93
7
6
2
Host
Rabbit
64
29
1
Clonality
Polyclonal
Conjugate
This CSF2RB antibody is un-conjugated
9
8
6
5
5
5
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
64
48
16
15
7
7
5
1
1
1
1
Supplier
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Catalog No. ABIN358962
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12226487 ABIN2789522 WB Rabbit C-Term Log in to see Polyclonal
0.12226487 ABIN2855774 FACS WB Rabbit IgG Center Log in to see Polyclonal
0.12226487 ABIN2705738 WB Rabbit pTyr593 Log in to see Polyclonal
0.12226487 ABIN2704591 WB Rabbit C-Term Log in to see Polyclonal
0.12226487 ABIN1858513 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 133-233 Log in to see Polyclonal
0.12226487 ABIN514676 WB Mouse AA 1-889, full length Log in to see Polyclonal
0.0011728628 ABIN358147 EIA WB Rabbit Ig pTyr766 Log in to see Polyclonal 5
0.0011728628 ABIN2878871 IHC (p) ELISA WB Rabbit IgG AA 559-608 Log in to see Polyclonal
0.0011728628 ABIN967466 FACS IP WB Mouse IgG1, kappa Log in to see 3D7 4
0.0011728628 ABIN205442 FACS IP WB Mouse IgG1 Log in to see 1C1
0.0011728628 ABIN799210 WB Rabbit C-Term Log in to see Polyclonal
0.0011728628 ABIN1822600 FACS IP WB Mouse IgG1, kappa Log in to see FIB504
0.0011728628 ABIN1733336 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0011728628 ABIN1975745 ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal
0.0011728628 ABIN1975746 ELISA WB PE Rabbit IgG N-Term Log in to see Polyclonal
0.0011728628 ABIN1983104 WB Mouse IgG1 AA 23-443 Log in to see MM0194-7D20
0.0011728628 ABIN1968851 ELISA WB Biotin Rabbit IgG N-Term Log in to see Polyclonal
0.0011728628 ABIN1968850 ELISA WB Biotin Rabbit IgG C-Term Log in to see Polyclonal
0.0011728628 ABIN1953253 DB ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Tyr766, pTyr766 Log in to see Polyclonal
0.0011728628 ABIN1954125 DB ELISA WB APC Rabbit IgG Tyr766, pTyr766 Log in to see Polyclonal

Top referenced anti-CSF2RB antibody for Western Blotting

Similar anti-CSF2RB Antibodies

Application / Reactivity Human
Dot Blot (DB) 7 Antibodies
ELISA 48 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 65 Antibodies

General

Antigen Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage) (CSF2RB) Antibodies
Epitope C-Term
(14), (11), (11), (9), (6), (6), (5), (5), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(93), (7), (6), (2)
Host Rabbit
(64), (29), (1)
Clonality
Polyclonal
Conjugate This CSF2RB antibody is un-conjugated
(9), (8), (6), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(64), (48), (16), (15), (7), (7), (5), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CSF2RB Antibody

Target Details CSF2RB Application Details Handling References for anti-CSF2RB Antibody (ABIN358962) Images
Specificity This antibody reacts to IL3R.
Purification Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal(V759) region of human IL3R.
Isotype Ig

Target Details CSF2RB

Product Details anti-CSF2RB Antibody Application Details Handling References for anti-CSF2RB Antibody (ABIN358962) Images back to top
Antigen
Alternative Name CD131 / CSF2RB (CSF2RB Antibody Abstract)
Background Purified rabbit polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.Synonyms: Cytokine receptor common subunit beta, GM-CSF-RB, GM-CSF/IL-3/IL-5 receptor common beta-chain, IL3RB, IL5RB
Gene ID 1439, 9606
UniProt P32927
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CSF2RB Antibody Target Details CSF2RB Handling References for anti-CSF2RB Antibody (ABIN358962) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CSF2RB Antibody Target Details CSF2RB Application Details References for anti-CSF2RB Antibody (ABIN358962) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CSF2RB Antibody (ABIN358962)

Product Details anti-CSF2RB Antibody Target Details CSF2RB Application Details Handling Images back to top
Background publications

Murphy, Ford, Olsen, Gustin, Jeffrey, Ollis, Young: "Interleukin-3 binding to the murine betaIL-3 and human betac receptors involves functional epitopes formed by domains 1 and 4 of different protein chains." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26500-8, 2004

McClure, Hercus, Cambareri, Woodcock, Bagley, Howlett, Lopez: "Molecular assembly of the ternary granulocyte-macrophage colony-stimulating factor receptor complex." in: Blood, Vol. 101, Issue 4, pp. 1308-15, 2003

Scibek, Evergren, Zahn, Canziani, Van Ryk, Chaiken: "Biosensor analysis of dynamics of interleukin 5 receptor subunit beta(c) interaction with IL5:IL5R(alpha) complexes." in: Analytical biochemistry, Vol. 307, Issue 2, pp. 258-65, 2002

Images

Product Details anti-CSF2RB Antibody Target Details CSF2RB Application Details Handling References for anti-CSF2RB Antibody (ABIN358962) back to top
Supplier Images
Western Blotting (WB) image for anti-CSF2RB antibody (Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage)) (C-Term) (ABIN358962) Western blot analysis of anti-IL3R Pab in HL60 cell line lysates (35ug/lane). IL3R (a...