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anti-Mouse (Murine) EPOR antibody for Immunocytochemistry

Recommended EPOR Antibody (supplied by: Log in to see )

Antigen
Erythropoietin Receptor (EPOR) Antibodies
  • EPOR
  • epor
  • EPO-R
  • erythropoietin receptor
  • Erythropoietin receptor
  • erythropoietin receptor homologue
  • erythropoietin receptor-like
  • EPOR
  • epor
  • Epor
  • LOC100355350
Epitope
AA 300-400, Internal Region
15
13
11
9
8
7
6
6
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
148
52
47
3
1
1
Host
Rabbit
114
33
14
Clonality
Polyclonal
Conjugate
This EPOR antibody is un-conjugated
10
7
4
4
4
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
112
75
27
24
16
16
14
13
5
2
1
1
1
Supplier
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Available images

Catalog No. ABIN446737
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1172012 ICC IHC IP WB Rabbit IgG AA 45-244 Log in to see Polyclonal
1 ABIN1843547 ICC IF WB Rabbit pTyr368 Log in to see Polyclonal
1 ABIN1843549 ICC IF WB Rabbit Log in to see Polyclonal
1 ABIN2857985 ICC IF WB Rabbit pTyr368 Log in to see Polyclonal
1 ABIN2858864 ICC IF WB Rabbit Log in to see Polyclonal

Similar anti-EPOR Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 19 Antibodies 20 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 6 Antibodies
Immunofluorescence (IF) 14 Antibodies 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 5 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 4 Antibodies
Immunoprecipitation (IP) 1 Antibodies 2 Antibodies
Simple Western (SimWes) 1 Antibodies 1 Antibodies
Western Blotting (WB) 32 Antibodies 37 Antibodies

General

Antigen Erythropoietin Receptor (EPOR) Antibodies
Epitope AA 300-400, Internal Region
(15), (13), (11), (9), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(148), (52), (47), (3), (1), (1)
Host Rabbit
(114), (33), (14)
Clonality
Polyclonal
Conjugate This EPOR antibody is un-conjugated
(10), (7), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(112), (75), (27), (24), (16), (16), (14), (13), (5), (2), (1), (1), (1)
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Product Details anti-EPOR Antibody

Target Details EPOR Application Details Handling Images
Specificity EpoR (A364) detects endogenous levels of EpoR protein.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal portion of the human EPO receptor protein (between residues 300-400) [UniProt P19235]

Target Details EPOR

Product Details anti-EPOR Antibody Application Details Handling Images back to top
Antigen
Alternative Name Erythropoietin receptor (EPOR Antibody Abstract)
Background Gene Symbol: EPOR
Gene ID 2057
UniProt P19235
Pathways JAK-STAT Signaling

Application Details

Product Details anti-EPOR Antibody Target Details EPOR Handling Images back to top
Application Notes Western Blot 2.0 μg/mL, Simple Western 1:10, Immunohistochemistry 1:50 - 1:100, Immunocytochemistry/Immunofluorescence 1:50 - 1:100, Immunohistochemistry-Paraffin 1:50-1:100In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot Protocol for EPO receptor antibody
Western Blot Protocol:
1. Perform SDS-PAGE on samples to be analyzed, loading 25 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute anti-HDAC5 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin protocol for EPO receptor antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-EPOR Antibody Target Details EPOR Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-EPOR Antibody Target Details EPOR Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-EPOR antibody (Erythropoietin Receptor) (AA 300-400) (ABIN446737) Immunocytochemistry/Immunofluorescence: Erythropoietin R Antibody [NBP1-19388] - EPO ...
Western Blotting (WB) image for anti-EPOR antibody (Erythropoietin Receptor) (AA 300-400) (ABIN446737) Western Blot: Erythropoietin R Antibody [NBP1-19388] - Western blot analysis of K562 ...
Simple Western (SimWes) image for anti-EPOR antibody (Erythropoietin Receptor) (AA 300-400) (ABIN446737) Simple Western: Erythropoietin R Antibody [NBP1-19388] - Simple Western lane view sho...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EPOR antibody (Erythropoietin Receptor) (AA 300-400) (ABIN446737) Immunohistochemistry-Paraffin: Erythropoietin R Antibody [NBP1-19388] - IHC analysis ...