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|Application / Reactivity||Mouse (Murine)||Human|
|Biochemical Assay (BCA)||2 Antibodies||2 Antibodies|
|ELISA||45 Antibodies||97 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||4 Antibodies||18 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies|
|Immunofluorescence (IF)||4 Antibodies|
|Immunohistochemistry (IHC)||19 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||15 Antibodies|
|Immunoprecipitation (IP)||12 Antibodies|
|Western Blotting (WB)||78 Antibodies|
|Antigen||FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies|
|Epitope||AA 7-176 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This FYN antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|10 references available|
|Supplier||Log in to see|
Product Details anti-FYN AntibodyTarget Details FYN Application Details Handling References for anti-FYN Antibody (ABIN94311) Images
|Specificity||The antibody FYN-01 reacts with Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase.|
|Purification||Purified from hybridoma culture supernatant by protein A-affinity chromatography.|
|Purity||> 95 % (by SDS-PAGE)|
|Immunogen||Bacterially expressed recombinant fragment of human Fyn (aa 7-176).|
Target Details FYNProduct Details anti-FYN Antibody Application Details Handling References for anti-FYN Antibody (ABIN94311) Images back to top
|Alternative Name||FYN (FYN Antibody Abstract)|
|Background||Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.|
|Pathways||JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-FYN Antibody Target Details FYN Handling References for anti-FYN Antibody (ABIN94311) Images back to top
Positive control: J77 T cell line, Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF)
Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
Western Blotting: Recommended dilution: 2 µg/mL
Positive control: JURKAT human T cell leukemia cell line
Immunohistochemistry (paraffin sections) Recommended dilution: 5 µg/mL
Positive tissue: brain
Working concentrations should be determined by the investigator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FYN Antibody Target Details FYN Application Details References for anti-FYN Antibody (ABIN94311) Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-FYN Antibody (ABIN94311)Product Details anti-FYN Antibody Target Details FYN Application Details Handling Images back to top
|Product cited in:||
Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011 (PubMed).
Meraner, Horejsí, Wolpl et al.: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, 2007 (PubMed).
Rivera, Olivera: "A current understanding of Fc epsilon RI-dependent mast cell activation." in: Current allergy and asthma reports, Vol. 8, Issue 1, pp. 14-20, 2008 (PubMed).
Filby, Seddon, Kleczkowska et al.: "Fyn regulates the duration of TCR engagement needed for commitment to effector function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 7, pp. 4635-44, 2007 (PubMed).
Wu, Hsu, Gleichman et al.: "Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2B cleavage in neurons and heterologous systems." in: The Journal of biological chemistry, Vol. 282, Issue 28, pp. 20075-87, 2007 (PubMed).
Makinodan, Manabe, Makinodan et al.: "A novel role for Fyn: change in sphere formation ability in murine embryonic stem cells." in: Neuroscience, Vol. 147, Issue 1, pp. 1-4, 2007 (PubMed).
Samayawardhena, Kapur, Craig: "Involvement of Fyn kinase in Kit and integrin-mediated Rac activation, cytoskeletal reorganization, and chemotaxis of mast cells." in: Blood, Vol. 109, Issue 9, pp. 3679-86, 2007 (PubMed).
Hattori, Uchino, Isosaka et al.: "Fyn is required for haloperidol-induced catalepsy in mice." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7129-35, 2006 (PubMed).
Talmor-Cohen, Tomashov-Matar, Tsai et al.: "Fyn kinase-tubulin interaction during meiosis of rat eggs." in: Reproduction (Cambridge, England), Vol. 128, Issue 4, pp. 387-93, 2004 (PubMed).
Utting, Priatel, Teh et al.: "p59fyn (Fyn) promotes the survival of anergic CD4-CD8- alpha beta TCR+ cells but negatively regulates their proliferative response to antigen stimulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 3, pp. 1540-6, 2001 (PubMed).
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