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anti-Mouse (Murine) FYN antibody for Immunofluorescence

Recommended FYN Antibody (supplied by: Log in to see )

Antigen
FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
  • AI448320
  • AW552119
  • c-fyn
  • cfyn-a
  • fyn
  • im:6908851
  • p59-FYN
  • PKC
  • si:dkey-119m7.5
  • SLK
  • SYN
  • zgc:86720
  • zgc:109996
Epitope
AA 7-176
56
33
20
20
15
8
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
223
124
39
7
7
7
2
1
1
1
1
Host
Mouse
132
99
16
Clonality
Monoclonal
Conjugate
This FYN antibody is un-conjugated
9
9
8
8
7
7
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
227
114
66
39
39
30
27
19
6
4
2
2
2
2
1
Supplier
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Catalog No. ABIN120706
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010840179 ABIN269149 ICC IF IHC (p) IP IHC WB Mouse IgG2b Log in to see FYN-01 3
0.0010840179 ABIN4312917 ICC IF IHC IHC (p) IP WB DyLight 350 Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312918 ICC IF IHC IHC (p) IP WB DyLight 405 Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312920 ICC IF IHC IHC (p) IP WB PerCP Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312921 ICC IF IHC IHC (p) IP WB Allophycocyanin Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312923 ICC IF IHC IHC (p) IP WB DyLight 680 Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312924 ICC IF IHC IHC (p) IP WB DyLight 488 Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312925 ICC IF IHC IHC (p) IP WB DyLight 405LS Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312926 ICC IF IHC IHC (p) IP WB Alexa Fluor 405 Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312928 ICC IF IHC IHC (p) IP WB Alexa Fluor 647 Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312929 ICC IF IHC IHC (p) IP WB Alexa Fluor 700 Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312919 ICC IF IHC IHC (p) IP WB PE Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312922 ICC IF IHC IHC (p) IP WB DyLight 755 Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN4312927 ICC IF IHC IHC (p) IP WB Alexa Fluor 488 Mouse IgG2b Log in to see FYN-01
0.0010840179 ABIN3038209 ICC IF IP IHC WB Mouse IgG2b AA 7-176 Log in to see
0.0010840179 ABIN3069359 ICC IF WB Mouse Log in to see

Top referenced anti-FYN antibody for Immunofluorescence

  • Human Monoclonal FYN Primary Antibody for ICC, IF - ABIN269149 (3 Publications): Taniguchi, Xia, Goldberg, Lee, Shah, Stavar, Masson, Momen, Shikatani, John, Husain, Fantus: Inhibition of Src kinase blocks high glucose-induced EGFR transactivation and collagen synthesis in mesangial cells and prevents diabetic nephropathy in mice. in Diabetes 2013 (PubMed)

Similar anti-FYN Antibodies

Application / Reactivity Mouse (Murine) Human
Biochemical Assay (BCA) 2 Antibodies 2 Antibodies
ELISA 45 Antibodies 99 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies 19 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Western Blotting (WB) 112 Antibodies

General

Antigen FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
Epitope AA 7-176
(56), (33), (20), (20), (15), (8), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(223), (124), (39), (7), (7), (7), (2), (1), (1), (1), (1)
Host Mouse
(132), (99), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FYN antibody is un-conjugated
(9), (9), (8), (8), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(227), (114), (66), (39), (39), (30), (27), (19), (6), (4), (2), (2), (2), (2), (1)
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Product Details anti-FYN Antibody

Target Details FYN Application Details Handling Images
Specificity The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (~59 kDa), which belongs to the Src family of cytoplasmic tyrosine kinases.
Purification Protein A Affinity Chromatography
Immunogen Bacterially expressed recombinant fragment of human Fyn (aa 7-176)
Clone FYN-01
Isotype IgG2b

Target Details FYN

Product Details anti-FYN Antibody Application Details Handling Images back to top
Antigen
Alternative Name FYN (FYN Antibody Abstract)
Background Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59 kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.Synonyms: Proto-oncogene tyrosine-protein kinase Fyn, Protooncogene Syn, SLK, p59-Fyn
Gene ID 2534
UniProt P06241
Pathways JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-FYN Antibody Target Details FYN Handling Images back to top
Application Notes Immunoprecipitation (Positive control: J77 T cell line, Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF). Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.) Western Blotting (2 mg/mL, Positive control: JURKAT human T cell leukemia cell line). Immunocytochemistry. Immunohistochemistry (paraffin sections): 5 m g/mL. Positive tissue: brain
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-FYN Antibody Target Details FYN Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM Sodium Azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. General.
Expiry Date 12 months