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|Application / Reactivity||Mouse (Murine)||Human|
|Biochemical Assay (BCA)||2 Antibodies||2 Antibodies|
|ELISA||45 Antibodies||99 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||4 Antibodies||19 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunocytochemistry (ICC)||20 Antibodies|
|Immunofluorescence (IF)||17 Antibodies|
|Immunohistochemistry (IHC)||33 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||29 Antibodies|
|Immunoprecipitation (IP)||26 Antibodies|
|Western Blotting (WB)||112 Antibodies|
|Antigen||FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies|
|Epitope||AA 7-176 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This FYN antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-FYN AntibodyTarget Details FYN Application Details Handling Images
|Specificity||The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (~59 kDa), which belongs to the Src family of cytoplasmic tyrosine kinases.|
|Purification||Protein A Affinity Chromatography|
|Immunogen||Bacterially expressed recombinant fragment of human Fyn (aa 7-176)|
Target Details FYNProduct Details anti-FYN Antibody Application Details Handling Images back to top
|Alternative Name||FYN (FYN Antibody Abstract)|
|Background||Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59 kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.Synonyms: Proto-oncogene tyrosine-protein kinase Fyn, Protooncogene Syn, SLK, p59-Fyn|
|Pathways||JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-FYN Antibody Target Details FYN Handling Images back to top
Immunoprecipitation (Positive control: J77 T cell line, Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF). Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.) Western Blotting (2 mg/mL, Positive control: JURKAT human T cell leukemia cell line). Immunocytochemistry. Immunohistochemistry (paraffin sections): 5 m g/mL. Positive tissue: brain
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FYN Antibody Target Details FYN Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM Sodium Azide, approx. pH 7.4|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. General.
|Expiry Date||12 months|