|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)||Dog (Canine)||Non Human Primates||Sheep (Ovine)|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||2 Antibodies||1 Antibodies|
|Western Blotting (WB)||94 Antibodies||15 Antibodies||4 Antibodies||3 Antibodies||16 Antibodies||1 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies||3 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies||11 Antibodies||4 Antibodies|
|Immunohistochemistry (IHC)||7 Antibodies||31 Antibodies||2 Antibodies|
|Antigen||Interferon (Alpha, beta and Omega) Receptor 1 (IFNAR1) Antibodies|
|Epitope||pSer539, pSer535 Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This IFNAR1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-IFNAR1 AntibodyTarget Details IFNAR1 Application Details Handling References for anti-IFNAR1 Antibody (ABIN372660) Images
|Specificity||This antibody is specific for the IFNAR1 protein phosphorylated at Ser535,539. Note: The Molecular Weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells, the mature form is ~110-130k.|
Species reactivity (expected):Bovine, Canine, Human, Mouse, Sheep and non-Human Primates.
Species reactivity (tested):Rat.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser535,539 of Human IFNAR1.|
Target Details IFNAR1Product Details anti-IFNAR1 Antibody Application Details Handling References for anti-IFNAR1 Antibody (ABIN372660) Images back to top
|Alternative Name||IFNAR1 (IFNAR1 Antibody Abstract)|
|Background||Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).Synonyms: CRF2-1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, IFN-R-1, IFN-alpha Receptor alpha, IFN-alpha/beta receptor 1, Interferon-alpha/beta receptor alpha chain, Type I interferon receptor 1|
|Research Area||Chromatin and Nuclear Signaling, Innate Immunity, Cytokines|
|Pathways||JAK-STAT Signaling, Hepatitis C|
Application DetailsProduct Details anti-IFNAR1 Antibody Target Details IFNAR1 Handling References for anti-IFNAR1 Antibody (ABIN372660) Images back to top
Western blot: 1/1000. Immunohistochemistry: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IFNAR1 Antibody Target Details IFNAR1 Application Details References for anti-IFNAR1 Antibody (ABIN372660) Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|
References for anti-IFNAR1 Antibody (ABIN372660)Product Details anti-IFNAR1 Antibody Target Details IFNAR1 Application Details Handling Images back to top
|Product cited in:||
Bhattacharya, HuangFu, Liu, Veeranki, Baker, Koumenis, Diehl, Fuchs: "Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2318-25, 2010
Kumar, Tang, Ravindranath, Clark, Croze, Fuchs: "SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor." in: The EMBO journal, Vol. 22, Issue 20, pp. 5480-90, 2003
Kirkwood: "Cancer immunotherapy: the interferon-alpha experience." in: Seminars in oncology, Vol. 29, Issue 3 Suppl 7, pp. 18-26, 2002
Biron: "Interferons alpha and beta as immune regulators--a new look." in: Immunity, Vol. 14, Issue 6, pp. 661-4, 2001
ImagesProduct Details anti-IFNAR1 Antibody Target Details IFNAR1 Application Details Handling References for anti-IFNAR1 Antibody (ABIN372660) back to top