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anti-Human IL-13 antibody for Blocking Reagent

Recommended IL-13 Antibody (supplied by: Log in to see )

Antigen
Interleukin 13 (IL13) Antibodies
  • Il-13
  • IL-13
  • IL13
  • L13
  • P600
Reactivity
Human
207
72
36
8
7
5
3
1
1
1
Host
Mouse
157
73
65
7
Clonality
Monoclonal
Conjugate
This IL-13 antibody is un-conjugated
40
17
9
6
5
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Neutralization (Neut), ELISA, Western Blotting (WB)
177
163
52
38
35
20
17
12
12
12
11
10
10
7
6
5
4
4
3
3
2
1
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Catalog No. ABIN250937
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General

Antigen Interleukin 13 (IL13) Antibodies
Reactivity Human
(207), (72), (36), (8), (7), (5), (3), (1), (1), (1)
Host Mouse
(157), (73), (65), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-13 antibody is un-conjugated
(40), (17), (9), (6), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Neutralization (Neut), ELISA, Western Blotting (WB)
(177), (163), (52), (38), (35), (20), (17), (12), (12), (12), (11), (10), (10), (7), (6), (5), (4), (4), (3), (3), (2), (1)
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Product Details anti-IL-13 Antibody

Target Details IL-13 Application Details Handling Images
Specificity Recognizes recombinant human IL-13 by capture ELISA and neutralization.
Cross-Reactivity (Details) Not yet tested in other species.
Purification Protein A purified
Immunogen Recombinant full length human IL13 protein expressed in E. coli.
Clone 32116-11
Isotype IgG1

Target Details IL-13

Product Details anti-IL-13 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-13 (IL13 Antibody Abstract)
Background Human IL-13 was originally identified by differential screening of an anti-CD28 activatedhuman peripheral blood mononuclear cell cDNA library as an induction-specific novelcytokine. It was also isolated from cDNA libraries of human T cell clones using themurine IL-13 (P600) cDNA as a probe.2 Human IL-13,a pleiotropic cytokine, is produced by activated Th0, Th1-like, Th2-like, and CD8 Tcells.1 The gene for human IL-13 maps to chromosome 5 and is closely linked to thegenes for IL-3, IL-4, IL-5, and GM-CSF.3 IL-13 inhibits proinflammatory cytokineproduction and stimulates antibody production.1 It induces proliferation in the humanpre-myeloid cell line TF-1. IL-13 hasmultiple effects on the differentiation and functions of monocytes and macrophages. Itsuppresses cytotoxic functions and induces changes in the morphology of humanmonocytes and in the phenotype of human monocytes and B cells by upregulating MHCclass II expression.4 IL-13 will also decrease the production of nitric oxide by activatedmurine macrophages, leading to impaired parasiticidal activity.5. Alternate Names: anti-ALRH antibody, anti-IL-13 antibody, anti-MGC116786 antibody, anti-MGC116788antibody, anti-MGC116789 antibody, anti-P600 antibody.
Gene Symbol: IL13
Gene ID 3596
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-13 Antibody Target Details IL-13 Handling Images back to top
Application Notes ELISA: Use in combination with a biotinylated IL13 polyclonal detection antibody at 400 ng/ml (100 ul/well), an ELISA for sample volumes of 100 ul with a range of 62.5-4000 µg/ml can be obtained. Neut: Use at a concentration of 1-3 µg/ml in the presence of 10 ng/ml of recombinant human IL13 using the human erythroleukemia cell line, TF-1. WB: Detects a band of approximately 9 kDa (predicted molecular weight: 12 kDa). The detection limit for recombinant human IL13 is approximately 50 ng/lane under non-reducing and reducing conditions. Endotoxin level is < 10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only

Handling

Product Details anti-IL-13 Antibody Target Details IL-13 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS, Trehalose
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.