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Human IL11 ELISA Kit

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Antigen
Interleukin 11 (IL11) ELISA Kits
  • AGIF
  • Il-11
  • IL-11
  • IL11
Epitope
AA 22-199
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
18
16
10
3
3
3
2
2
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN1889298
$ 333.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN414873 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.30628014 ABIN1379967 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.30628014 ABIN1115603 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.30628014 ABIN2708258 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN365225 Sandwich ELISA Serum, Cell Culture Supernatant, Urine, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012155735 ABIN3032940 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 1.1-800 pg/mL 1.1 pg/mL Log in to see
0.0012155735 ABIN626496 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012155735 ABIN455522 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN625008 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 4
0.0012155735 ABIN3199223 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 62.50-4000 pg/mL 62.50 pg/mL Log in to see
0.0012155735 ABIN2684566 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4-1600 pg/mL 4 pg/mL Log in to see 3
0.0012155735 ABIN1028958 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012155735 ABIN2114257 Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.0012155735 ABIN2116068 40-1000 pg/mL 40 pg/mL Log in to see
0.0012155735 ABIN577100 Sandwich ELISA Log in to see
0.0012155735 ABIN2535341 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012155735 ABIN2088599 Sandwich ELISA Log in to see
0.0012155735 ABIN2948711 31.25-2000 pg/mL 31.25 pg/mL Log in to see

Top referenced IL11 ELISA Kit for ELISA

Similar IL11 ELISA Kits

Application / Reactivity Human
ELISA 19 ELISA Kits

General

Antigen Interleukin 11 (IL11) ELISA Kits
Epitope AA 22-199
Reactivity Human
Kits with alternative reactivity to:
(18), (16), (10), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 3 references available
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Product Details IL11 ELISA Kit

Target details Application Details Handling References for IL11 Kit (ABIN1889298) Images
Purpose For quantitative detection of human IL-11 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant human IL-11
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human IL-11 antibod - 1
  • Lyophilized recombinant human IL-11 standar - 10ng/tubex2
  • Biotinylated anti- human IL-11 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: P22-L199

Target details

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Antigen
Alternative Name IL11 (IL11 ELISA Kit Abstract)
Background Interleukin 11(IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.
Gene ID 3589
UniProt P20809
Research Area Stem Cells, Hematopoietic Progenitors, Cytokines, Innate Immunity, Adaptive Immunity, Angiogenesis
Pathways JAK-STAT Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL-11 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-11 has been precoated onto 96-well plates. Standards(E.coli, P22-L199) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-11 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-11 standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 70, Standard deviation: 3.22, CV(%): 4.6
  • Sample 2: n=16, Mean(pg/ml): 460, Standard deviation: 23.92, CV(%): 5.2
  • Sample 3: n=16, Mean(pg/ml): 1327, Standard deviation: 71.66, CV(%): 5.4,
  • Sample 1: n=24, Mean(pg/ml): 93, Standard deviation: 5.487, CV(%): 5.9
  • Sample 2: n=24, Mean(pg/ml): 554, Standard deviation: 36.01, CV(%): 6.5
  • Sample 3: n=24, Mean(pg/ml): 1395, Standard deviation: 94.86, CV(%): 6.8
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL11 Kit (ABIN1889298)

Product Details IL11 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Sims, Jenkins, Nakamura, Quinn, Li, Gillespie, Ernst, Robb, Martin: "Interleukin-11 receptor signaling is required for normal bone remodeling." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 20, Issue 7, pp. 1093-102, 2005

Takeuchi, Watanabe, Ishii, Takeda, Nakayama, Fukumoto, Kaneta, Inoue, Matsumoto, Harigaya, Fujita: "Interleukin-11 as a stimulatory factor for bone formation prevents bone loss with advancing age in mice." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 49011-8, 2002

McKinley, Wu, Yang-Feng, Yang: "Genomic sequence and chromosomal location of human interleukin-11 gene (IL11)." in: Genomics, Vol. 13, Issue 3, pp. 814-9, 1992

Images

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Supplier Images
ELISA image for IL11 ELISA Kit (Interleukin 11) (ABIN1889298) Human IL-11 PicoKine ELISA Kit standard curve