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anti-Human IL12 antibody for ELISA (Detection)

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • CLMF
  • CLMF2
  • IL-12 p40
  • Il-12a
  • IL-12B
  • IL-12p35
  • IL-12p40
  • Il12
  • il12b
  • IL12B
  • il12p40.b
  • Ll12a
  • LOC100217488
  • NKSF
  • NKSF2
  • p35
  • zgc:152789
Reactivity
Human
160
63
16
5
3
2
2
2
1
1
1
Host
Mouse
115
46
42
36
2
1
Clonality
Monoclonal
Conjugate
This IL12 antibody is conjugated to Biotin
31
13
10
8
7
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA (Detection)
165
155
44
33
28
22
11
10
9
9
8
7
7
6
5
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN279993
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087659294 ABIN1721168 ELISA (Detection) Biotin Mouse IgG1 Log in to see B-P24
0.00087659294 ABIN1721167 ELISA (Detection) ELISPOT (Detection) Biotin Mouse IgG1 Log in to see B-P24

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General

Antigen Interleukin 12 (IL12) Antibodies
Reactivity Human
(160), (63), (16), (5), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(115), (46), (42), (36), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12 antibody is conjugated to Biotin
(31), (13), (10), (8), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Detection)
(165), (155), (44), (33), (28), (22), (11), (10), (9), (9), (8), (7), (7), (6), (5), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling Images
Specificity The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.
Characteristics The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically- related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.
Synonyms: Synonyms: Interleukin-12/Interleukin-23 p40/70
Clone C8-6
Isotype IgG1 kappa

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-12 p40 /70 (IL12 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling Images back to top
Application Notes Applications reported: The C8.6 antibody has been reported for use in ELISA.

Applications tested: The biotinylated C8.6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the purified B-T21 (13-7128) antibody for capture and recombinant human IL-12 p70 (14-8129) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5 - 2.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate. The biotinylated C8.6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-12 p40 monomer in combination with the purified C8.3 (14-7127) antibody for capture and recombinant human IL-12 p40 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 10000 pg/mL - 80 pg/mL should be included in each ELISA plate.
Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze.
Storage 4 °C