IL12 antibody (Biotin)

Catalog No. ABIN6963864

Product Details anti-IL12 Antibody (Biotin)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL12 antibody is conjugated to Biotin

Application: ELISA, ELISpot

Specificity: IL-12 (p70)

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-12 (p70) from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of human IL-12 (p70) in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-12 p70

Clone: MT704

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963864 and ABIN7448307 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAbs .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL12 antibody (Biotin) (ABIN6963864)

Tomasicchio, Semple, Esmail, Meldau, Randall, Pooran, Davids, Cairncross, Anderson, Downs, Malherbe, Novitzky, Panieri, Oelofse, Londt, Naiker, Dheda: "An autologous dendritic cell vaccine polarizes a Th-1 response which is tumoricidal to patient-derived breast cancer cells." in: Cancer immunology, immunotherapy : CII, Vol. 68, Issue 1, pp. 71-83, (2019) (PubMed).

Tootoonchian, Pak, Ardekani, Sehati, Abedi-Valugerdi, Kokhaei: "Evaluation of interleukin 12 and CD56+ lymphocyte cells in pediatric hematopoietic stem cell transplantation for early diagnosis of acute graft versus host disease." in: Transplant immunology, Vol. 39, pp. 25-29, (2018) (PubMed).

Tagawa, Albanese, Bouvet, Moosmann, Mautner, Heissmeyer, Zielinski, Lutter, Hoser, Hastreiter, Hayes, Sugden, Hammerschmidt: "Epstein-Barr viral miRNAs inhibit antiviral CD4+ T cell responses targeting IL-12 and peptide processing." in: The Journal of experimental medicine, Vol. 213, Issue 10, pp. 2065-80, (2017) (PubMed).

Zhang, Morgan, Beane, Zheng, Dudley, Kassim, Nahvi, Ngo, Sherry, Phan, Hughes, Kammula, Feldman, Toomey, Kerkar, Restifo, Yang, Rosenberg: "Tumor-infiltrating lymphocytes genetically engineered with an inducible gene encoding interleukin-12 for the immunotherapy of metastatic melanoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 21, Issue 10, pp. 2278-88, (2016) (PubMed).

Persson, Ekerfelt, Jablonowska, Jonsson, Ernerudh, Jenmalm, Berg: "Immunological status in patients undergoing in vitro fertilisation: responses to hormone treatment and relationship to outcome." in: Journal of reproductive immunology, Vol. 96, Issue 1-2, pp. 58-67, (2013) (PubMed).

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL12 (p70) (IL12 Products)

Synonyms: CLMF antibody, CLMF2 antibody, IL-12B antibody, NKSF antibody, NKSF2 antibody, p40 antibody, Il-12b antibody, Il12p40 antibody, Il-12p40 antibody, Il12 antibody, IL-12p40 antibody, IL-12 antibody, LOC100217488 antibody, IL-12p35 antibody, Il-12a antibody, Ll12a antibody, p35 antibody, IL12p40 antibody, IL12B antibody, IL-12 p35 antibody, interleukin 12B antibody, interleukin 12A antibody, interleukin 12b antibody, interleukin 12a antibody, Interleukin 12 level antibody, IL12B antibody, IL12A antibody, Il12b antibody, Il12a antibody, IL12 antibody, il12a antibody

Gene ID: 3592, 3593

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome