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anti-Human IL12 antibody for Blocking Antibody

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • CLMF
  • CLMF2
  • IL-12 p40
  • Il-12a
  • IL-12B
  • IL-12p35
  • IL-12p40
  • Il12
  • il12b
  • IL12B
  • il12p40.b
  • Ll12a
  • LOC100217488
  • NKSF
  • NKSF2
  • p35
  • zgc:152789
Reactivity
Human
159
63
16
5
3
2
2
2
1
1
1
Host
Goat
116
44
42
36
2
1
Clonality
Polyclonal
Conjugate
This IL12 antibody is un-conjugated
32
13
10
8
7
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
164
153
44
32
28
22
11
10
9
8
8
7
7
6
5
4
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1002587
$ 374.22
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00086286204 ABIN284527 Inhibition ELISA WB Mouse IgG1 kappa Log in to see M12
0.00086286204 ABIN1002584 Inhibition Mouse IgG1 Log in to see
0.00086286204 ABIN636601 Inhibition ELISA WB Goat Log in to see Polyclonal
0.00086286204 ABIN2689734 Inhibition ELISA WB Rat IgG1, kappa Log in to see 20C2

Similar anti-IL12 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 5 Antibodies
ELISA 102 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 7 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 26 Antibodies
Functional Studies (Func) 11 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 23 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Neutralization (Neut) 15 Antibodies

General

Antigen Interleukin 12 (IL12) Antibodies
Reactivity Human
(159), (63), (16), (5), (3), (2), (2), (2), (1), (1), (1)
Host Goat
(116), (44), (42), (36), (2), (1)
Clonality
Polyclonal
Conjugate This IL12 antibody is un-conjugated
(32), (13), (10), (8), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(164), (153), (44), (32), (28), (22), (11), (10), (9), (8), (8), (7), (7), (6), (5), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling Images
Purification Antigen affinity purified polyclol antibody
Sterility Sterile filtered
Immunogen recombint hIL-12 (human Interleukin-12)
Isotype IgG

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 12 (IL12 Antibody Abstract)
Background Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-12 (human Interleukin-12). Anti-Human IL-12 specific antibody was purified by affinity chromatography employing immobilized hIL-12 matrix.
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling Images back to top
Application Notes To yield one-half maximal inhibition [ND₅₀] of the biological activity of hIL-12 (0.3 ng/µl), a concentration of 0.012 - 0.02 µg/µl of this antibody is required. To detect hIL-12 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. To detect hIL-12 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human IL-12 (PK-AB815-62212GB) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA. To detect hIL-12 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human IL-12 (PK-AB815-62212GB) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details Images back to top
Format Lyophilized
Concentration To yield one-half maximal inhibition [ND₅₀] of the biological activity of hIL-12 (0.3 ng/µl), a concentration of 0.012 - 0.02 µg/µl of this antibody is required.
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handling Advice The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Storage -20 °C
Expiry Date 24 months