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anti-Human IL12 antibody for Blocking Antibody

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • IL-12 p40
  • il12b
  • il12p40.b
  • zgc:152789
  • LOC100217488
  • IL-12B
  • IL12B
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL-12p40
  • interleukin 12Ba
  • interleukin 12B
  • interleukin 12 p40 subunit
  • interleukin 12a
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • il12ba
  • LOC100217488
  • IL-12
  • Il12a
  • IL12A
  • Il12b
  • IL12B
Reactivity
Human
160
62
16
9
5
2
2
2
1
1
1
Host
Mouse
110
44
44
33
2
1
Clonality
Monoclonal
Conjugate
This IL12 antibody is un-conjugated
32
13
8
8
7
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition)
166
154
66
41
29
19
15
13
10
7
6
6
5
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN2689729
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1002587 Inhibition ELISA WB Goat IgG Log in to see Polyclonal
1 ABIN284527 Inhibition ELISA WB Mouse IgG1 kappa Log in to see M12
1 ABIN636601 Inhibition ELISA WB Goat Log in to see Polyclonal
1 ABIN1002584 Inhibition Mouse IgG1 Log in to see
1 ABIN2689734 Inhibition ELISA WB Rat IgG1, kappa Log in to see 20C2

Top referenced anti-IL12 antibody for Blocking Antibody

Similar anti-IL12 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 6 Antibodies
Blocking Peptide (BP) 1 Antibodies
ELISA 106 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Functional Studies (Func) 12 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 53 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 1 Antibodies

General

Antigen Interleukin 12 (IL12) Antibodies
Reactivity Human
(160), (62), (16), (9), (5), (2), (2), (2), (1), (1), (1)
Host Mouse
(110), (44), (44), (33), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12 antibody is un-conjugated
(32), (13), (8), (8), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition)
(166), (154), (66), (41), (29), (19), (15), (13), (10), (7), (6), (6), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling References for anti-IL12 Antibody (ABIN2689729) Images
Brand BD Pharmingen™
Characteristics The C11.5 antibody reacts with human IL-12 p40 monomer and p70 heterodimer, but not p35 monomer. The immunogen used to generate the C11.5 hybridoma was the CHO-expressed recombinant human IL-12 p70 heterodimer. p40 has also been described as a subunit of IL-23 and thus it is possible that the C11.5 antibody will crossreact with IL-23. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of IL-12 p40/p70 by activated CD14+ human PBMCs. Ficoll-separated human PBMCs were primed for 2 hrs with recombinant human IFN-γ (10 ng/mL, Cat. No. 554616), then activated with IFN-γ (10 ng/mL) and LPS (100 ng/mL, Sigma) in the presence of 2 μM GolgiStop™ (Cat. No. 554724) for an additional 22 hr. Cells were harvested, stained with FITC-mouse anti human CD14 antibody (Cat. No. 555397), fixed, permeabilized, and then stained with 0.125 μg of PE-C11.5 antibody (Cat. No. 554575), following Pharmingen's staining protocol (left panel). The data reflect gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, the binding of the PE-C11.5 antibody was blocked by preincubation of the fixed/permeabilized cells with the unlabeled C11.5 antibody (5 μg, Cat. No. 554573) prior to staining (right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.

BD Pharmingen™ Purified Mouse Anti-Human IL-12 (p40/p70) - Purified - Clone C11.5 - Isotype Mouse IgG1 - Reactivity Hu - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen CHO-expressed recombinant human IL-12 p70 heterodimer
Clone C11-5
Isotype IgG1

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling References for anti-IL12 Antibody (ABIN2689729) Images back to top
Antigen
Alternative Name IL-12 p40,p70 (IL12 Antibody Abstract)
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling References for anti-IL12 Antibody (ABIN2689729) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details References for anti-IL12 Antibody (ABIN2689729) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-IL12 Antibody (ABIN2689729)

Product Details anti-IL12 Antibody Target Details IL12 Application Details Handling Images back to top
Product cited in:

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." in: Immunity, Vol. 13, Issue 5, pp. 715-25, 2001

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, 1992