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anti-Mouse (Murine) IL12 antibody for Blocking Antibody

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • CLMF
  • CLMF2
  • IL-12 p40
  • Il-12a
  • IL-12B
  • IL-12p35
  • IL-12p40
  • Il12
  • il12b
  • IL12B
  • il12p40.b
  • Ll12a
  • LOC100217488
  • NKSF
  • NKSF2
  • p35
  • zgc:152789
Reactivity
Mouse (Murine)
161
65
16
5
3
2
2
2
1
1
1
Host
Rat
117
45
43
31
2
1
Clonality
Monoclonal
Conjugate
This IL12 antibody is un-conjugated
32
13
9
8
7
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition)
166
154
44
28
28
20
12
10
9
9
8
7
6
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2689731
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000870507 ABIN1002588 Inhibition ELISA WB Goat IgG Log in to see Polyclonal
0.000870507 ABIN1002586 Inhibition ELISA Rat IgG1 kappa Log in to see
0.000870507 ABIN933784 Inhibition ELISA WB Rat IgG1 kappa Log in to see M912296
0.000870507 ABIN636602 Inhibition ELISA WB Goat Log in to see Polyclonal

Top referenced anti-IL12 antibody for Blocking Antibody

Similar anti-IL12 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 49 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (IHC) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Immunostaining (ISt) 2 Antibodies
Neutralization (Neut) 14 Antibodies

General

Antigen Interleukin 12 (IL12) Antibodies
Reactivity Mouse (Murine)
(161), (65), (16), (5), (3), (2), (2), (2), (1), (1), (1)
Host Rat
(117), (45), (43), (31), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12 antibody is un-conjugated
(32), (13), (9), (8), (7), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition)
(166), (154), (44), (28), (28), (20), (12), (10), (9), (9), (8), (7), (6), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling References for anti-IL12 Antibody (ABIN2689731) Images
Brand BD Pharmingen™
Characteristics The C15.6 antibody reacts with both free and complexed (homodimer p80 and heterodimer p70) forms of the p40 subunit of mouse interleukin-12 (IL-12). The immunogen used to generate the C15.6 hybridoma was recombinant mouse IL-12 p70 protein. p40 has also been described as a subunit of IL-23 and thus it is possible that the C15.6 antibody will crossreact with IL-23. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of IL-12 by mouse bone marrow-derived macrophages. Bone marrow cells from 6 month old BALB/c mice were cultured for 10 days in mouse GM-CSF (40 ng/mL final concentration, Cat. No. 554586). Adherent cells were washed and treated for ~14 hours with mouse IFN-γ (10 ng/mL final concentration, Cat. No. 554587), subsequently LPS (1 μg/mL final concentration, Sigma) and GolgiStop™ (2 μM final concentration Cat. No. 554724) were added to cultures for an additional 5 hours. Adherent cells were washed and then incubated with 1x trypsin EDTA at 37 °C. for 15 minutes and gently dislodged by pipetting. Nonspecific surface binding was blocked by incubation of cells with purified polyclonal normal mouse immunoglobulin. Cells were then fixed, permeabilized, and non-specific binding to intracellular antigens was blocked using PBS/2 % BSA/0.1 % saponin. Cells were then stained with 0.06 μg of PE-conjugated rat anti-mouse IL-12 (p40/p70) antibody (PE-C15.6, Cat. No. 554479, see left panel) using the BD Pharmingen™ staining protocol. To demonstrate specificity of staining, the binding of PE-C15.6 antibody was blocked by the preincubation of the conjugated antibody with recombinant mouse IL-12 p40 protein (0.5 μg, Cat. No. 554594, see middle panel), and by preincubating the fixed/permeabilized cells with unlabeled C15.6 antibody (5 μg/mL final concentration, Cat. No. 554477, see right panel) prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and verified using the ligand blocking and unlabeled antibody blocking controls.

BD Pharmingen™ Purified Rat Anti-Mouse IL-12 (p40/p70) - Purified - Clone C15.6 - Isotype Rat IgG1 - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen CHO-expressed recombinant mouse IL-12 p70 protein
Clone C15-6
Isotype IgG1

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling References for anti-IL12 Antibody (ABIN2689731) Images back to top
Antigen
Alternative Name IL-12 p40,p70 (IL12 Antibody Abstract)
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling References for anti-IL12 Antibody (ABIN2689731) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details References for anti-IL12 Antibody (ABIN2689731) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-IL12 Antibody (ABIN2689731)

Product Details anti-IL12 Antibody Target Details IL12 Application Details Handling Images back to top
Product cited in:

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Wysocka, Kubin, Vieira, Ozmen, Garotta, Scott, Trinchieri: "Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice." in: European journal of immunology, Vol. 25, Issue 3, pp. 672-6, 1995