Horse (Equine) IL12 ELISA Kit

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Antigen
Interleukin 12 (IL12) ELISA Kits
  • IL-12 p40
  • il12b
  • il12p40.b
  • zgc:152789
  • LOC100217488
  • IL-12B
  • IL12B
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL-12p40
  • interleukin 12Ba
  • interleukin 12B
  • interleukin 12 p40 subunit
  • interleukin 12a
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • il12ba
  • LOC100217488
  • IL-12
  • Il12a
  • IL12A
  • Il12b
  • IL12B
Reactivity
Horse (Equine)
Alternatives
Kits with alternative reactivity to:
74
36
12
10
9
8
7
7
6
5
5
5
3
2
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
31.25 pg/mL - 2000 pg/mL
Minimum Detection Limit
31.25 pg/mL
Application
ELISA
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Catalog No. ABIN5655759
$ 844.25
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN2535361 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN4970347 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see

General

Antigen Interleukin 12 (IL12) ELISA Kits
Reactivity Horse (Equine)
Kits with alternative reactivity to:
(74), (36), (12), (10), (9), (8), (7), (7), (6), (5), (5), (5), (3), (2), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 31.25 pg/mL - 2000 pg/mL
Minimum Detection Limit 31.25 pg/mL
Application ELISA
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Product Details IL12 ELISA Kit

Target details Application Details Handling Images
Sample Type Biological Fluids, Plasma, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This assay has high sensitivity and excellent specificity for detection of Interleukin 12A (IL12A). No significant cross-reactivity or interference between Interleukin 12A (IL12A) and analogues was observed.
Sensitivity 13.3 pg/mL

Target details

Product Details IL12 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 12A (IL12 ELISA Kit Abstract)
Background

Gene Name: Interleukin 12A

Gene Aliases: p35, IL12-A, CLMF1, NFSK, NKSF1, Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, NF Cell Stimulatory Factor Chain 1

Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details IL12 ELISA Kit Target details Handling Images back to top
Comment

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time 3 h
Plate Pre-coated
Protocol The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12A (IL12A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12A (IL12A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12A (IL12A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 12A (IL12A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12A (IL12A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12A (IL12A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only

Handling

Product Details IL12 ELISA Kit Target details Application Details Images back to top
Handling Advice The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Storage 4 °C,-20 °C
Storage Comment -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Expiry Date 4-8 months