|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||8 Antibodies|
|Blocking Peptide (BP)||2 Antibodies|
|Dot Blot (DB)||4 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||24 Antibodies|
|Flow Cytometry (FACS)||105 Antibodies|
|Functional Studies (Func)||16 Antibodies|
|Immunoassay (IA)||3 Antibodies|
|Immunocytochemistry (ICC)||26 Antibodies|
|Immunofluorescence (IF)||17 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies|
|Immunohistochemistry (IHC)||58 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Antigen||Interleukin 2 (IL2) Antibodies|
|Conjugate||This IL2 antibody is un-conjugated Alternatives|
ELISA (Capture), Enzyme Immunoassay (EIA)
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Product Details anti-IL2 AntibodyTarget Details IL2 Application Details Handling Images
|Specificity||Reactive to the recombinant Human IL2.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Characteristics||Synonyms: IL-2, TCGF, T-cell growth factor|
|Purification||Affinity Chromatography on Protein G|
|Immunogen||Recombinant Human Interleukin-2 (IL2)|
Target Details IL2Product Details anti-IL2 Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-2 / IL2 (IL2 Antibody Abstract)|
|Background||Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF|
|Pathways||JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation|
Application DetailsProduct Details anti-IL2 Antibody Target Details IL2 Handling Images back to top
ELISA: Clone 9F9 can be used as Capture antibody to detect recombinant Human IL-2. Thisclone matches with HRP conjugated clone 5A8, clone 10C4 and clone 6B1. When used reversely as detection antibody, 9F9 also matches with 5A8, 10C5 and 6B1. However, clone 9F9 produced better results when it is used as Capture antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL2 Antibody Target Details IL2 Application Details Images back to top
|Reconstitution||Restore with double distillated water is recommended to adjust the final concentration to 1.0 mg/mL|
|Buffer||0.01 M PBS, pH 7.2|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|