anti-Human IL2 antibody for Intracellular Staining

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • IL2
  • IL-2
  • il2
  • TCGF
  • lymphokine
  • Il-2
  • POIL2
  • interleukin 2
  • IL2
  • il2
  • Il2
Reactivity
Human
465
241
98
31
17
10
6
6
5
4
3
3
1
1
Host
Rat
314
261
211
17
5
4
4
Clonality
Monoclonal
Conjugate
This IL2 antibody is un-conjugated
86
49
49
28
19
18
17
14
12
11
8
8
7
7
7
7
7
7
7
7
6
6
6
4
4
4
4
4
4
4
3
3
2
2
2
1
1
Application
Intracellular Staining (ICS), Neutralization (Neut), ELISA
455
387
219
86
71
49
48
48
38
35
26
23
15
11
10
7
7
6
4
4
3
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN1176901
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Top referenced anti-IL2 antibody for Intracellular Staining

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General

Antigen Interleukin 2 (IL2) Antibodies
Reactivity Human
(465), (241), (98), (31), (17), (10), (6), (6), (5), (4), (3), (3), (1), (1)
Host Rat
(314), (261), (211), (17), (5), (4), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL2 antibody is un-conjugated
(86), (49), (49), (28), (19), (18), (17), (14), (12), (11), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Application Intracellular Staining (ICS), Neutralization (Neut), ELISA
(455), (387), (219), (86), (71), (49), (48), (48), (38), (35), (26), (23), (15), (11), (10), (7), (7), (6), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling ProductDetails: References for anti-IL2 antibody (ABIN1176901) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-2. Neutralization activity may be measured with a proliferation assay using 100 pg/mL recombinant human IL-2 (Cat. No. 554603) to stimulate CTLL-2 cells at 2x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human IL-2 for 60 minutes).
50% Neutralization (ND50) at 0.1-1.5 µg/mL
>95% Neutralization at 3 - 15 myg/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Human IL-2 Recombinant Protein
Clone MQ1-17H12
Isotype IgG2a, kappa

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling ProductDetails: References for anti-IL2 antibody (ABIN1176901) Images back to top
Antigen
Alternative Name IL-2 (IL2 Antibody Abstract)
Background The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2). The immunogen used to generate the MQ1-17H12 hybridoma was recombinant human IL-2. Unconjugated or purified forms of this antibody have been reported to be neutralizing for human IL-2 bioactivity.
Research Area Cancer
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling ProductDetails: References for anti-IL2 antibody (ABIN1176901) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details ProductDetails: References for anti-IL2 antibody (ABIN1176901) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

ProductDetails: References for anti-IL2 antibody (ABIN1176901)

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
Product cited in:

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Andersson, Abrams, Björk, Funa, Litton, Agren, Andersson: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995

Fernandez, Andersson, Andersson, Troye-Blomberg: "Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid." in: European journal of immunology, Vol. 24, Issue 8, pp. 1808-15, 1994

Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, 1978