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anti-Mouse (Murine) IL2 antibody for ELISpot

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • Il-2
  • IL-2
  • IL2
  • il2
  • lymphokine
  • POIL2
  • TCGF
Reactivity
Mouse (Murine)
472
257
89
20
18
7
6
4
4
3
2
2
1
1
Host
Rat
297
260
243
17
7
4
Clonality
Monoclonal
Conjugate
This IL2 antibody is un-conjugated
95
51
50
28
21
16
15
12
12
9
7
7
7
7
7
7
7
7
6
6
6
6
5
5
4
4
4
4
4
2
2
2
2
2
1
1
1
1
Application
ELISA (Capture), ELISpot, Immunoprecipitation (IP), Neutralization (Neut)
470
384
219
69
65
55
45
43
38
36
30
27
20
15
13
8
7
6
5
4
4
4
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN282505
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008779775 ABIN1177352 ELISpot Log in to see
0.0008779775 ABIN1448867 ELISpot Log in to see

Top referenced anti-IL2 antibody for ELISpot

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General

Antigen Interleukin 2 (IL2) Antibodies
Reactivity Mouse (Murine)
(472), (257), (89), (20), (18), (7), (6), (4), (4), (3), (2), (2), (1), (1)
Host Rat
(297), (260), (243), (17), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL2 antibody is un-conjugated
(95), (51), (50), (28), (21), (16), (15), (12), (12), (9), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application ELISA (Capture), ELISpot, Immunoprecipitation (IP), Neutralization (Neut)
(470), (384), (219), (69), (65), (55), (45), (43), (38), (36), (30), (27), (20), (15), (13), (8), (7), (6), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN282505) Images
Specificity The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The JES6-1A12 antibody is a neutralizing antibody. Mouse IL-2 (also known as TCGF) is a 17 kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions, and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.
Characteristics The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The JES6-1A12 antibody is a neutralizing antibody. Mouse IL-2 (also known as TCGF) is a 17 kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non- specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions, and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.
Synonyms: Synonyms: Interleukin-2
Purification Affinity Purified
Endotoxin Level <0.001 ng endotoxin/μg
Grade Functional Grade
Clone JES6-1A12
Isotype IgG2a kappa

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling References for anti-IL2 Antibody (ABIN282505) Images back to top
Antigen
Alternative Name IL-2 (IL2 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling References for anti-IL2 Antibody (ABIN282505) Images back to top
Application Notes Applications reported: The JES6-1A12 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-2 bioactivity, and IP.

Applications tested: The Functional Grade Purified JES6-1A12 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-2 bioactivity. The JES6-1A12 antibody at 0.250 μg/mL has been found to inhibit by 50 % the biological effects of 1.0 ng/mL mouse IL-2 in a CTLL-2 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. The JES6-1A12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-2 (IL-2) in combination with the biotin JES6-5H4 (13-7021) antibody for detection and recombinant mouse IL-2 (14-8021) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.
Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details References for anti-IL2 Antibody (ABIN282505) Images back to top
Concentration 1 mg/mL
Buffer aqueous buffer, no sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Use in sterile environment.
Storage 4 °C

References for anti-IL2 Antibody (ABIN282505)

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
Background publications

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Images

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN282505) back to top
Supplier Images
 image for anti-IL2 antibody (Interleukin 2) (ABIN282505) Splenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 w...
 image for anti-IL2 antibody (Interleukin 2) (ABIN282505) anti-Interleukin 2 (IL2) antibody (Image 2)