anti-Mouse (Murine) IL2 antibody for Functional Studies

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • IL2
  • IL-2
  • il2
  • TCGF
  • lymphokine
  • Il-2
  • POIL2
  • interleukin 2
  • IL2
  • il2
  • Il2
Reactivity
Mouse (Murine)
482
247
98
31
17
10
6
6
5
4
3
3
1
1
Host
Rabbit
321
274
214
17
5
4
4
Clonality
Polyclonal
Conjugate
This IL2 antibody is un-conjugated
89
51
51
28
21
18
17
14
12
11
9
8
8
7
7
7
7
7
7
7
6
6
6
4
4
4
4
4
4
4
3
3
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
467
391
227
93
72
49
49
48
41
40
29
25
15
11
10
8
7
7
5
4
3
3
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN115873
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115874 EIA Func WB Rabbit Log in to see Polyclonal 1
1 ABIN264017 Func Rat IgG Log in to see I2-C7
1 ABIN2474962 Func ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN641023 Func ELISA WB Rabbit IgG Log in to see

Top referenced anti-IL2 antibody for Functional Studies

Similar anti-IL2 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 120 Antibodies
ELISpot 2 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
FLISA 2 Antibodies
Flow Cytometry (FACS) 85 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies

General

Antigen Interleukin 2 (IL2) Antibodies
Reactivity Mouse (Murine)
(482), (247), (98), (31), (17), (10), (6), (6), (5), (4), (3), (3), (1), (1)
Host Rabbit
(321), (274), (214), (17), (5), (4), (4)
Clonality
Polyclonal
Conjugate This IL2 antibody is un-conjugated
(89), (51), (51), (28), (21), (18), (17), (14), (12), (11), (9), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
(467), (391), (227), (93), (72), (49), (49), (48), (41), (40), (29), (25), (15), (11), (10), (8), (7), (7), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling ProductDetails: References for anti-IL2 antibody (ABIN115873) Images
Specificity Detects Murine IL-2
Purification Affinity chromatography
Immunogen Highly pure (>98%) recombinant mIL-2

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling ProductDetails: References for anti-IL2 antibody (ABIN115873) Images back to top
Antigen
Alternative Name Interleukin-2 / IL2 (IL2 Antibody Abstract)
Background Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF
Gene ID 16183
NCBI Accession NP_032392
UniProt P04351
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling ProductDetails: References for anti-IL2 antibody (ABIN115873) Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (2.0 ng/mL), aconcentration of 0.04-0.05 μg/mL of this antibody is required. ELISA: (Indirect): To detect mIL-2 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mIL-2. (Sandwich): To detect mIL-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine IL-2 (PP009B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-2. Western Blot: To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details ProductDetails: References for anti-IL2 antibody (ABIN115873) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C under subdued light for one month or (in aliquots) at -20 °C for longer. This product is phototsensitive and should be protected from light.

ProductDetails: References for anti-IL2 antibody (ABIN115873)

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
Background publications

Huse, Lillemeier, Kuhns, Chen, Davis: "T cells use two directionally distinct pathways for cytokine secretion." in: Nature immunology, Vol. 7, Issue 3, pp. 247-55, 2006