anti-Mouse (Murine) IL2 antibody for Western Blotting

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • IL2
  • IL-2
  • il2
  • TCGF
  • lymphokine
  • Il-2
  • POIL2
  • interleukin 2
  • IL2
  • il2
  • Il2
Reactivity
Mouse (Murine)
482
247
98
31
17
10
6
6
5
4
3
3
1
1
Host
Rat
321
275
213
17
5
4
4
Clonality
Monoclonal
Conjugate
This IL2 antibody is un-conjugated
89
51
51
28
21
18
17
14
12
11
9
8
8
7
7
7
7
7
7
7
6
6
6
4
4
4
4
4
4
4
3
3
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
467
391
227
93
72
49
49
47
41
40
29
26
15
11
10
8
7
7
5
4
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN119367
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.347841 ABIN4324782 IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 2
3.347841 ABIN3043201 WB Rabbit IgG AA 151-169, C-Term Log in to see Polyclonal 4
3.347841 ABIN254448 IP ELISA WB Rat IgG2a Log in to see JES6-1A12
3.347841 ABIN2474971 IP ELISA WB Rat IgG2a Log in to see JES6-1A12 4
3.347841 ABIN2706379 WB Rabbit Center Log in to see Polyclonal
3.347841 ABIN2736966 WB Mouse Log in to see
3.347841 ABIN2692436 ELISA WB Rabbit IgG AA 21-169 Log in to see Polyclonal
3.347841 ABIN1868617 ICC IHC IP WB Rabbit IgG AA 10-169 Log in to see Polyclonal
3.347841 ABIN3031467 IHC ELISA WB Rabbit Ig Fraction AA 50-77 Log in to see Polyclonal
3.347841 ABIN3028759 WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN707321 IF (p) IHC (p) WB Rabbit IgG AA 80-130 Log in to see Polyclonal
1 ABIN1177301 Neut WB Rat IgG2a Log in to see S4B6 3
1 ABIN673149 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN115873 EIA Func WB Rabbit Log in to see Polyclonal 1
1 ABIN115874 EIA Func WB Rabbit Log in to see Polyclonal 1
1 ABIN707330 IHC (p) WB HRP Rabbit IgG AA 80-130 Log in to see Polyclonal
1 ABIN465129 Neut ELISA WB Rat IgG2a Log in to see
1 ABIN217638 IP ELISA WB Rat IgG2a Log in to see
1 ABIN115871 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN115872 EIA WB Biotin Rabbit Log in to see Polyclonal

Top referenced anti-IL2 antibody for Western Blotting

Similar anti-IL2 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 120 Antibodies
ELISpot 2 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
FLISA 2 Antibodies
Flow Cytometry (FACS) 85 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies

General

Antigen Interleukin 2 (IL2) Antibodies
Reactivity Mouse (Murine)
(482), (247), (98), (31), (17), (10), (6), (6), (5), (4), (3), (3), (1), (1)
Host Rat
(321), (275), (213), (17), (5), (4), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL2 antibody is un-conjugated
(89), (51), (51), (28), (21), (18), (17), (14), (12), (11), (9), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(467), (391), (227), (93), (72), (49), (49), (47), (41), (40), (29), (26), (15), (11), (10), (8), (7), (7), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling ProductDetails: References for anti-IL2 antibody (ABIN119367) Images
Specificity This antibody is specific for Interleukin-2 (IL-2). This antibody recognises both natural and recombinant mouse IL-2. Clone JES6-1A12 is reported to function as a neutralizing antibody. We recommends the use of SM1698A for this purpose.
Purification Affinity chromatography
Immunogen E. coli expressed recombinant mouse IL-2.
Clone JES6-1A12
Isotype IgG2a

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling ProductDetails: References for anti-IL2 antibody (ABIN119367) Images back to top
Antigen
Alternative Name Interleukin-2 / IL2 (IL2 Antibody Abstract)
Background Murine IL-2 is a potent lymphoid cell growth factor which primarily acts on T lymphocytes.Synonyms: IL-2, T-cell growth factor, TCGF
Gene ID 16183
NCBI Accession NP_032392
UniProt P04351
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling ProductDetails: References for anti-IL2 antibody (ABIN119367) Images back to top
Application Notes ELISA: 1 μg/mL - 4 μg/mL. Immunoprecipitation. Western Blot: 1 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details ProductDetails: References for anti-IL2 antibody (ABIN119367) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-IL2 antibody (ABIN119367)

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
Background publications

Sander, Höidén, Andersson, Möller, Abrams: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Images

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling ProductDetails: References for anti-IL2 antibody (ABIN119367) back to top
Supplier Images
Western Blotting (WB) image for anti-IL2 antibody (Interleukin 2) (ABIN119367) Western blot analysis of mouse IL-2 recombinant protein probed with RAT ANTI MOUSE IN...