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anti-Cow (Bovine) IL21 antibody for Enzyme Immunoassay

Recommended IL21 Antibody (supplied by: Log in to see )

Antigen
Interleukin 21 (IL21) Antibodies
  • IL-21
  • Za11
Epitope
AA 24-152
25
14
13
10
8
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine)
161
66
21
6
4
4
Host
Rabbit
155
33
9
8
4
Clonality
Polyclonal
Conjugate
This IL21 antibody is un-conjugated
18
13
9
8
7
6
4
3
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
125
122
20
15
15
13
11
8
7
6
6
5
4
3
2
1
1
1
1
1
1
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Catalog No. ABIN401062
$ 500.50
Plus shipping costs $45.00

Top referenced anti-IL21 antibody for Enzyme Immunoassay

Similar anti-IL21 Antibodies

Application / Reactivity Cow (Bovine)
ELISA 6 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Western Blotting (WB) 7 Antibodies

General

Antigen Interleukin 21 (IL21) Antibodies
Epitope AA 24-152
(25), (14), (13), (10), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(161), (66), (21), (6), (4), (4)
Host Rabbit
(155), (33), (9), (8), (4)
Clonality
Polyclonal
Conjugate This IL21 antibody is un-conjugated
(18), (13), (9), (8), (7), (6), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(125), (122), (20), (15), (15), (13), (11), (8), (7), (6), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL21 Antibody

Target Details IL21 Application Details Handling References for anti-IL21 Antibody (ABIN401062) Images
Specificity This antibody reacts to Interleukin-21.
Cross-Reactivity (Details) Species reactivity (expected):Porcine, Horse, Human, Canine, Macaque, Platypus and Syrian hamster, Mouse, Rat, Chicken.
Species reactivity (tested):Bovine.
Purification Affinity chromatography on Protein A
Immunogen Recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein
Isotype IgG

Target Details IL21

Product Details anti-IL21 Antibody Application Details Handling References for anti-IL21 Antibody (ABIN401062) Images back to top
Antigen
Alternative Name Interleukin-21 / IL21 (IL21 Antibody Abstract)
Background Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common ?-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Ra chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells' (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells, with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81 % and 58-67 % homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MATSynonyms: IL-21, Za11
Gene ID 378475
NCBI Accession NP_942129
UniProt Q76LU5
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL21 Antibody Target Details IL21 Handling References for anti-IL21 Antibody (ABIN401062) Images back to top
Application Notes ELISA: 1/6,000. Western Blot: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL21 Antibody Target Details IL21 Application Details References for anti-IL21 Antibody (ABIN401062) Images back to top
Format Liquid
Concentration 1.0 mg/mL by UV absorbance at 280 nm
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-IL21 Antibody (ABIN401062)

Product Details anti-IL21 Antibody Target Details IL21 Application Details Handling Images back to top
Background publications

Muneta, Kikuma, Yoshihara, Mori: "Cloning, expression, and tissue distribution of bovine interleukin-21." in: Veterinary immunology and immunopathology, Vol. 95, Issue 1-2, pp. 73-80, 2003

Images

Product Details anti-IL21 Antibody Target Details IL21 Application Details Handling References for anti-IL21 Antibody (ABIN401062) back to top
Supplier Images
 image for anti-IL21 antibody (Interleukin 21) (AA 24-152) (ABIN401062) anti-Interleukin 21 (IL21) (AA 24-152) antibody