IL-21 antibody (Biotin)

Catalog No. ABIN6964032

Product Details anti-IL-21 Antibody (Biotin)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-21 antibody is conjugated to Biotin

Application: ELISA

Specificity: Native and recombinant human IL-21

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-21

Clone: MT21-3m

Isotype: IgG1

Application Details

Application Notes: To retain detectable levels of IL-21 in cell culture supernatants, biotinylated mAb MT21.3m can be included during cell culture. The antibody will bind to secreted IL-21 thus preventing IL-21 from interacting with its receptor. In this way, IL-21 is kept in solution and can be quantified in ELISA. For this purpose, MT21.3m is free of preservatives. Please note that mAb MT21.3m is optimized for use with human IL-21 ELISA kits and anti-human IL-21 mAbs from Mabtech.

Comment:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS free of preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-21 antibody (Biotin) (ABIN6964032)

Laestadius, Ingelman-Sundberg, Myrberg, Verme, Sundberg, Schweiger, Saghafian-Hedengren, Nilsson: "Altered proportions of circulating CXCR5+ helper T cells do not dampen influenza vaccine responses in children with rheumatic disease." in: Vaccine, Vol. 37, Issue 28, pp. 3685-3693, (2020) (PubMed).

Yamamoto, Kitawaki, Sugimoto, Fujita, Kawase, Takaori-Kondo, Kadowaki: "Anti-inflammatory modulation of human myeloid-derived dendritic cell subsets by lenalidomide." in: Immunology letters, Vol. 211, pp. 41-48, (2020) (PubMed).

Lasaviciute, Björkander, Carvalho-Queiroz, Hed Myrberg, Nussbaum, Nilsson, Bemark, Nilsson, Sverremark-Ekström, Saghafian-Hedengren: "Epstein-Barr Virus, but Not Cytomegalovirus, Latency Accelerates the Decay of Childhood Measles and Rubella Vaccine Responses-A 10-Year Follow-up of a Swedish Birth Cohort." in: Frontiers in immunology, Vol. 8, pp. 1865, (2017) (PubMed).

Amu, Lantto Graham, Bekele, Nasi, Bengtsson, Rethi, Sorial, Meini, Zazzi, Hejdeman, Chiodi: "Dysfunctional phenotypes of CD4+ and CD8+ T cells are comparable in patients initiating ART during early or chronic HIV-1 infection." in: Medicine, Vol. 95, Issue 23, pp. e3738, (2017) (PubMed).

Huang, Ehrnfelt, Paulie, Zuber, Ahlborg: "ELISpot and ELISA analyses of human IL-21-secreting cells: Impact of blocking IL-21 interaction with cellular receptors." in: Journal of immunological methods, Vol. 417, pp. 60-66, (2015) (PubMed).

Target Details for IL-21

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL21 (IL21 Products)

Synonyms: IL-21 antibody, Za11 antibody, interleukin 21 antibody, IL21 antibody, Il21 antibody

Gene ID: 59067

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response