You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Cow (Bovine) IL21 antibody for ELISA

Recommended IL21 Antibody (supplied by: Log in to see )

Antigen
Interleukin 21 (IL21) Antibodies
  • IL-21
  • Za11
Epitope
AA 24-152
25
14
13
10
8
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Pig (Porcine), Horse (Equine)
161
66
21
6
3
3
Host
Rabbit
155
33
9
8
4
Clonality
Polyclonal
Conjugate
This IL21 antibody is un-conjugated
18
13
9
8
7
6
4
3
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
125
121
20
15
15
13
11
8
7
6
6
5
4
3
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN964710
$ 135.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012205833 ABIN964709 ELISA WB Rabbit IgG AA 24-152 Log in to see Polyclonal
0.0012205833 ABIN1102475 ELISA WB Rabbit IgG AA 24-152 Log in to see Polyclonal
0.0012205833 ABIN2291384 ELISA WB Rabbit IgG AA 24-152 Log in to see Polyclonal
0.0012205833 ABIN2487538 ELISA WB Rabbit AA 24-152 Log in to see Polyclonal
0.0012205833 ABIN2487537 ELISA WB Rabbit AA 24-152 Log in to see Polyclonal

Similar anti-IL21 Antibodies

Application / Reactivity Cow (Bovine) Pig (Porcine) Horse (Equine)
Western Blotting (WB) 7 Antibodies 4 Antibodies 4 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
ELISA 6 Antibodies 4 Antibodies 4 Antibodies

General

Antigen Interleukin 21 (IL21) Antibodies
Epitope AA 24-152
(25), (14), (13), (10), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Pig (Porcine), Horse (Equine)
(161), (66), (21), (6), (3), (3)
Host Rabbit
(155), (33), (9), (8), (4)
Clonality
Polyclonal
Conjugate This IL21 antibody is un-conjugated
(18), (13), (9), (8), (7), (6), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(125), (121), (20), (15), (15), (13), (11), (8), (7), (6), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-IL21 Antibody

Target Details IL21 Application Details Handling Images
Specificity This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined.
Cross-Reactivity Pig (Porcine), Horse (Equine)
Characteristics Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common gamma-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Ralpha chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells’ (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells, with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81% and 58-67% homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MAT. Anti-IL21 antibody is ideal for investigators involved in Cancer and Immunology research.
Purification purified
Sterility Sterile filtered
Immunogen This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details IL21

Product Details anti-IL21 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL21 (IL21 Antibody Abstract)
Background Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common γ-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Rα chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells’ (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells; with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81% and 58-67% homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MAT. Anti-IL21 antibody is ideal for investigators involved in Cancer and Immunology research.
Synonyms: Interleukin-21, IL-21
Gene ID 378475
UniProt Q76LU5
Research Area Cancer, Cytokines, Innate Immunity, Immunology, Virology, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL21 Antibody Target Details IL21 Handling Images back to top
Application Notes IL-21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes, and in HL-60 and THP-1 cell lines. This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.1 kDa in size corresponding to bovine IL-21 protein by western blotting in the appropriate cell lysate or extract.
Comment

Gene Name: IL21

Restrictions For Research Use only

Handling

Product Details anti-IL21 Antibody Target Details IL21 Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.
Expiry Date 3 months

Images

Product Details anti-IL21 Antibody Target Details IL21 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL21 antibody (Interleukin 21) (AA 24-152) (ABIN964710) Western blot using Protein-A Purified Anti-bovine IL-21 antibody shows detection of r...