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anti-Mouse (Murine) IL21 antibody for Intracellular Flow Cytometry

Recommended IL21 Antibody (supplied by: Log in to see )

Antigen
Interleukin 21 (IL21) Antibodies
  • IL-21
  • Za11
Reactivity
Mouse (Murine)
197
79
21
7
4
4
Host
Rat
156
68
21
9
5
Clonality
Monoclonal
Conjugate
This IL21 antibody is conjugated to Alexa Fluor 647
20
16
9
9
8
6
6
4
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
176
123
34
15
15
13
11
8
7
6
6
5
4
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2657956
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008684246 ABIN2666224 ICFC Biotin Mouse IgG1 kappa Log in to see 7H20-I19-M3 4
0.0008684246 ABIN2666225 ICFC Mouse IgG1 kappa Log in to see 5H3-O12-L23 4

Top referenced anti-IL21 antibody for Intracellular Flow Cytometry

Similar anti-IL21 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 40 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 47 Antibodies

General

Antigen Interleukin 21 (IL21) Antibodies
Reactivity Mouse (Murine)
(197), (79), (21), (7), (4), (4)
Host Rat
(156), (68), (21), (9), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL21 antibody is conjugated to Alexa Fluor 647
(20), (16), (9), (9), (8), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(176), (123), (34), (15), (15), (13), (11), (8), (7), (6), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-IL21 Antibody

Target Details IL21 Application Details Handling References for anti-IL21 Antibody (ABIN2657956) Images
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Clone BL25168
Isotype IgG2b

Target Details IL21

Product Details anti-IL21 Antibody Application Details Handling References for anti-IL21 Antibody (ABIN2657956) Images back to top
Antigen
Alternative Name IL-21 (IL21 Antibody Abstract)
Background IL-21 is a 17 kD member of the common γ chain family of cytokines and mainly produced by CD4+ T subsets, including Th17, follicular helper T cells (TFH), Th2, memory CD4+ T cells, and NKT cells. IL-21 signals through a heterodimeric receptor complex containing IL-21R and the common cytokine receptor γ chain (CD132). IL-21 has effects on T, B, NK, and myeloid cells, regulates both humoral and cell-mediated immunity. It has been reported that IL-21 is able to enhance the expansion of Th17 cells via the induction of IL-23R, cooperates with IL-7 or IL-15 to promote CD8+ T cell expansion. IL-21 is also known to regulate Ig production by driving the differentiation of B cells to antibody-producing plasma cells and involving in the development of follicular helper T cells (TFH). In addition, IL-21 exerts negative effect on lymphoid and myeloid cells, including induction of B cell apoptosis and inhibition of dendritic cell maturation and function. The type of actions mediated by IL-21 is presumably determined by its biological context, specific activation state of the target cells, as well as the cytokine milieu. IL-21/IL-21R system is involved in the pathogenesis of various autoimmune diseases and anti-tumor effects.
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL21 Antibody Target Details IL21 Handling References for anti-IL21 Antibody (ABIN2657956) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL21 Antibody Target Details IL21 Application Details References for anti-IL21 Antibody (ABIN2657956) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-IL21 Antibody (ABIN2657956)

Product Details anti-IL21 Antibody Target Details IL21 Application Details Handling Images back to top
Background publications

Cedeno-Laurent, Opperman, Barthel, Kuchroo, Dimitroff: "Galectin-1 triggers an immunoregulatory signature in Th cells functionally defined by IL-10 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 7, pp. 3127-37, 2012

Bauquet, Jin, Paterson, Mitsdoerffer, Ho, Sharpe, Kuchroo: "The costimulatory molecule ICOS regulates the expression of c-Maf and IL-21 in the development of follicular T helper cells and TH-17 cells." in: Nature immunology, Vol. 10, Issue 2, pp. 167-75, 2009

Pot, Jin, Awasthi, Liu, Lai, Madan, Sharpe, Karp, Miaw, Ho, Kuchroo: "Cutting edge: IL-27 induces the transcription factor c-Maf, cytokine IL-21, and the costimulatory receptor ICOS that coordinately act together to promote differentiation of IL-10-producing Tr1 cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 2, pp. 797-801, 2009

Spolski, Kim, Zhu, Levy, Leonard: "IL-21 mediates suppressive effects via its induction of IL-10." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 5, pp. 2859-67, 2009

Suto, Kashiwakuma, Kagami, Hirose, Watanabe, Yokote, Saito, Nakayama, Grusby, Iwamoto, Nakajima: "Development and characterization of IL-21-producing CD4+ T cells." in: The Journal of experimental medicine, Vol. 205, Issue 6, pp. 1369-79, 2008

Spolski, Leonard: "Interleukin-21: basic biology and implications for cancer and autoimmunity." in: Annual review of immunology, Vol. 26, pp. 57-79, 2008

Korn, Bettelli, Gao, Awasthi, Jäger, Strom, Oukka, Kuchroo: "IL-21 initiates an alternative pathway to induce proinflammatory T(H)17 cells." in: Nature, Vol. 448, Issue 7152, pp. 484-7, 2007

Asao, Okuyama, Kumaki, Ishii, Tsuchiya, Foster, Sugamura: "Cutting edge: the common gamma-chain is an indispensable subunit of the IL-21 receptor complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 1, pp. 1-5, 2001

Images

Product Details anti-IL21 Antibody Target Details IL21 Application Details Handling References for anti-IL21 Antibody (ABIN2657956) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL21 antibody (Interleukin 21)  (Alexa Fluor 647) (ABIN2657956) anti-Interleukin 21 (IL21) antibody (Alexa Fluor 647)
Flow Cytometry (FACS) image for anti-IL21 antibody (Interleukin 21)  (Alexa Fluor 647) (ABIN2657956) anti-Interleukin 21 (IL21) antibody (Alexa Fluor 647) (Image 2)