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Mouse (Murine) IL21 ELISA Kit

Recommended IL21 Product (supplied by: Log in to see )

Antigen
Interleukin 21 (IL21) ELISA Kits
  • IL-21
  • Za11
  • interleukin 21
  • interleukin 21-like
  • IL21
  • Il21
  • LOC100344172
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
24
23
13
4
3
3
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
6-2000 pg/mL
Minimum Detection Limit
6 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625144
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
11.490515 ABIN1672800 Sandwich ELISA Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see 4
11.490515 ABIN457134 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
11.490515 ABIN424205 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
11.490515 ABIN3198574 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.25-2000 pg/mL 31.25 pg/mL Log in to see
11.490515 ABIN1115465 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
11.490515 ABIN2708550 Sandwich ELISA Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN4993129 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 8.2-2000 pg/mL 8.2 pg/mL Log in to see
1 ABIN1081676 Sandwich ELISA Serum, Plasma, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
1 ABIN511098 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see
1 ABIN1379957 Sandwich ELISA Cell Lysate, Serum, Plasma 46-3000 pg/mL 46 pg/mL Log in to see
1 ABIN1570703 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN4368197 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Body Fluids 23-1500 pg/mL 23 pg/mL Log in to see
1 ABIN4368200 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Body Fluids 23-1500 pg/mL 23 pg/mL Log in to see
1 ABIN2114301 Cell Lysate, Serum, Plasma 46-3000 pg/mL 46 pg/mL Log in to see
1 ABIN2042538 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN4947760 Sandwich ELISA Log in to see
1 ABIN2684660 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum Log in to see
1 ABIN2535496 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
1 ABIN4970426 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN455566 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced IL21 ELISA Kit for ELISA

Similar IL21 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 23 ELISA Kits

General

Antigen Interleukin 21 (IL21) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(24), (23), (13), (4), (3), (3), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 6-2000 pg/mL
Minimum Detection Limit 6 pg/mL
Application ELISA
Pubmed 4 references available
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Product Details IL21 ELISA Kit

Target details Application Details Handling References for IL21 Kit (ABIN625144) Images
Purpose Mouse IL-21 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Serum, Plasma, Cell Culture Supernatant
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivity < 6 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IL21 ELISA Kit Application Details Handling References for IL21 Kit (ABIN625144) Images back to top
Antigen
Alternative Name IL-21 (IL21 ELISA Kit Abstract)
Background The Mouse IL-21 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-21 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gene ID 60505
UniProt E9PX58
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details IL21 ELISA Kit Target details Handling References for IL21 Kit (ABIN625144) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of cell culture supernatants. Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture supernates) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 40 µL IL-21 standard (50 ng/mL) from the vial of Item C, into a tube with 960 µL Assay Diluent A or 1x Assay Diluent B to prepare a 2,000 pg/mL standard solution. Pipette 300 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the 2,000 pg/mL standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 40 µL standard + 960 µL 200 µL 200 µL 200 µL 200 µL 200 µL 200myl 2,000 800 320 128 51.20 20.48 8.19 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 200-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 10 ml 1x Assay Diluent B to prepare a 200-fold diluted HRP- Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Mouse IL-21 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Mouse IL-21 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10
Sensitivity: The minimum detectable dose of IL-21 is typically less than 6 pg/mL.
Recovery: Recovery was determined by spiking various levels of mouse IL-21 into mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 93.57 83-104 Plasma 77.38 68-87 Cell culture media 98.65 87-109
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 110.3 106.7 115.0 Range ( %) 101-119 84-103 104-124 1:4 Average % of Expected 105.8 103.7 123.5 Range ( %) 96-114 92-112 111-124
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details IL21 ELISA Kit Target details Application Details References for IL21 Kit (ABIN625144) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for IL21 Kit (ABIN625144)

Product Details IL21 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Su, Duan, Liang, Feng-Wang, Zheng, Fu, Yan, Ling-Huang, Wang: "Lycium barbarum polysaccharides as an adjuvant for recombinant vaccine through enhancement of humoral immunity by activating Tfh cells." in: Veterinary immunology and immunopathology, Vol. 158, Issue 1-2, pp. 98-104, 2014

Su, Duan, Liang, Feng-Wang, Zheng, Fu, Yan, Ling-Huang, Wang: "Lycium barbarum polysaccharides as an adjuvant for recombinant vaccine through enhancement of humoral immunity by activating Tfh cells." in: Veterinary immunology and immunopathology, Vol. 158, Issue 1-2, pp. 98-104, 2014

Su, Duan, Liang, Feng-Wang, Zheng, Fu, Yan, Ling-Huang, Wang: "Lycium barbarum polysaccharides as an adjuvant for recombinant vaccine through enhancement of humoral immunity by activating Tfh cells." in: Veterinary immunology and immunopathology, Vol. 158, Issue 1-2, pp. 98-104, 2014

Su, Duan, Liang, Feng-Wang, Zheng, Fu, Yan, Ling-Huang, Wang: "Lycium barbarum polysaccharides as an adjuvant for recombinant vaccine through enhancement of humoral immunity by activating Tfh cells." in: Veterinary immunology and immunopathology, Vol. 158, Issue 1-2, pp. 98-104, 2014

Images

Product Details IL21 ELISA Kit Target details Application Details Handling References for IL21 Kit (ABIN625144) back to top
Supplier Images
ELISA image for IL21 ELISA Kit (Interleukin 21) (ABIN625144) Interleukin 21 (IL21) ELISA Kit