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Mouse (Murine) IL21 ELISA Kit

Recommended IL21 Product (supplied by: Log in to see )

Antigen
Interleukin 21 (IL21) ELISA Kits
  • IL-21
  • Za11
Epitope
AA 25-146
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
25
22
12
4
4
3
3
2
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN1672800
$ 399.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN424205 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32693866 ABIN1115465 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32693866 ABIN2708550 Sandwich ELISA Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.32693866 ABIN1033724 Sandwich ELISA Log in to see
0.0012117105 ABIN457134 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN625144 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant Log in to see 4
0.0012117105 ABIN3198574 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012117105 ABIN511098 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see
0.0012117105 ABIN1379957 Sandwich ELISA Cell Lysate, Serum, Plasma 46-3000 pg/mL 46 pg/mL Log in to see
0.0012117105 ABIN1570703 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN2042538 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN2114301 Cell Lysate, Serum, Plasma 46-3000 pg/mL 46 pg/mL Log in to see
0.0012117105 ABIN2684660 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum Log in to see
0.0012117105 ABIN1033885 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (EDTA) Log in to see
0.0012117105 ABIN2535496 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012117105 ABIN455566 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2088777 Sandwich ELISA Log in to see
0.0012117105 ABIN2948773 31.25-2000 pg/mL 31.25 pg/mL Log in to see

Top referenced IL21 ELISA Kit for ELISA

Similar IL21 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 19 ELISA Kits

General

Antigen Interleukin 21 (IL21) ELISA Kits
Epitope AA 25-146
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(25), (22), (12), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 4 references available
Supplier Log in to see

Product Details IL21 ELISA Kit

Target details Application Details Handling References for IL21 Kit (ABIN1672800) Images
Purpose For quantitative detection of mouse IL-21 in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant mouse IL-21
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse IL-21antibod - 1
  • Lyophilized recombinant mouse IL-21 standar - 10ng/tubex2
  • Biotinylated anti- mouse IL-21 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: P25-S146

Target details

Product Details IL21 ELISA Kit Application Details Handling References for IL21 Kit (ABIN1672800) Images back to top
Antigen
Alternative Name IL21 (IL21 ELISA Kit Abstract)
Background Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer(NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells.
Gene ID 60505
UniProt Q5SUE2
Research Area Cancer, Cytokines, Innate Immunity, Immunology, Virology, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details IL21 ELISA Kit Target details Handling References for IL21 Kit (ABIN1672800) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse IL-21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-21 has been precoated onto 96-well plates. Standards(E.coli, P25-S146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-21 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-21 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 316, Standard deviation: 15.8, CV(%): 5
  • Sample 2: n=16, Mean(pg/ml): 2196, Standard deviation: 80.4, CV(%): 6.2
  • Sample 3: n=16, Mean(pg/ml): 2113, Standard deviation: 99.31, CV(%): 4.7,
  • Sample 1: n=24, Mean(pg/ml): 427, Standard deviation: 25.2, CV(%): 5.9
  • Sample 2: n=24, Mean(pg/ml): 1450, Standard deviation: 97.15, CV(%): 6.7
  • Sample 3: n=24, Mean(pg/ml): 2485, Standard deviation: 159, CV(%): 6.4
Restrictions For Research Use only

Handling

Product Details IL21 ELISA Kit Target details Application Details References for IL21 Kit (ABIN1672800) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL21 Kit (ABIN1672800)

Product Details IL21 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Yu, Tian, Wang, Guo, Li, Wang, Li, Zhang, Zhuang, Ling: "An HIV-1 envelope immunogen with W427S mutation in CD4 binding site induced more T follicular helper memory cells and reduced non-specific antibody responses." in: PLoS ONE, Vol. 9, Issue 12, pp. e115047, 2014

Background publications

Nurieva, Yang, Martinez, Zhang, Panopoulos, Ma, Schluns, Tian, Watowich, Jetten, Dong: "Essential autocrine regulation by IL-21 in the generation of inflammatory T cells." in: Nature, Vol. 448, Issue 7152, pp. 480-3, 2007

Mehta, Wurster, Whitters, Young, Collins, Grusby: "IL-21 induces the apoptosis of resting and activated primary B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 8, pp. 4111-8, 2003

Parrish-Novak, Dillon, Nelson, Hammond, Sprecher, Gross, Johnston, Madden, Xu, West, Schrader, Burkhead, Heipel, Brandt, Kuijper, Kramer, Conklin, Presnell, Berry, Shiota, Bort, Hambly, Mudri, Clegg et al.: "Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. ..." in: Nature, Vol. 408, Issue 6808, pp. 57-63, 2000

Images

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Supplier Images
ELISA image for IL21 ELISA Kit (Interleukin 21) (ABIN1672800) Mouse IL-21 PicoKine ELISA Kit standard curve