Rabbit IL21 ELISA Kit

Recommended IL21 Product (supplied by: Log in to see )

Antigen
Interleukin 21 (IL21) ELISA Kits
  • IL-21
  • Za11
  • interleukin 21
  • interleukin 21-like
  • IL21
  • Il21
  • LOC100344172
Reactivity
Rabbit
Alternatives
Kits with alternative reactivity to:
25
24
14
5
3
3
2
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
2.45-600 ng/mL
Minimum Detection Limit
2.45 ng/mL
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN2748242
$ 556.60
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN628350 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see

General

Antigen Interleukin 21 (IL21) ELISA Kits
Reactivity Rabbit
Kits with alternative reactivity to:
(25), (24), (14), (5), (3), (3), (2), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 2.45-600 ng/mL
Minimum Detection Limit 2.45 ng/mL
Application ELISA
Supplier Log in to see

Product Details IL21 ELISA Kit

Target details Application Details Handling Images
Purpose Rabbit IL-21 ELISA Kit for serum, plasma, and cell culture supernatant samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rabbit IL-1alpha, IL-1beta, IL-8, IL-17A, Leptin, MIP-1beta, MMP-9, NCAM-1, TNF alpha
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IL21 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IL-21 (IL21 ELISA Kit Abstract)
Background IL-21
UniProt G1T948
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details IL21 ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details IL21 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months