anti-Human IL3RA antibody for Immunocytochemistry

Recommended IL3RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
  • Cyrl
  • CD123
  • IL3R
  • IL3RAY
  • IL3RX
  • IL3RY
  • hIL-3Ra
  • CDw123
  • SUT-1
  • interleukin 3 receptor, alpha
  • interleukin 3 receptor, alpha (low affinity)
  • interleukin 3 receptor, alpha chain
  • Il3ra
  • IL3RA
Epitope
AA 20-305
16
15
13
11
11
10
8
8
6
5
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
251
56
4
Host
Mouse
160
99
41
2
Clonality
Monoclonal
Conjugate
This IL3RA antibody is un-conjugated
25
23
20
12
11
8
7
5
5
5
5
5
5
4
4
4
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
183
149
61
46
31
26
26
24
14
13
9
8
8
5
5
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4899495
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.59048 ABIN4899494 FACS ICC IHC Neut WB Mouse IgG1 AA 20-305 Log in to see 32703 2
27.59048 ABIN1724855 ICC FACS IHC ELISA WB Mouse IgG1 AA 200-305 Log in to see 8E11C5 2
27.59048 ABIN1724866 ICC FACS ELISA WB Mouse IgG1 AA 200-305 Log in to see 10B8E7 2
1 ABIN4880790 ELISA FACS ICC IHC WB Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN4325125 CyTOF ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 8E11C5
1 ABIN4880335 ELISA FACS ICC WB Mouse IgG1 AA 200-305 Log in to see 10B8E7
1 ABIN4325124 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 10B8E7
1 ABIN1174728 ICC IHC IP WB Rabbit IgG AA 19-157 Log in to see Polyclonal
1 ABIN1823498 ICC FACS IHC (p) ELISA WB Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN1905181 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 AA 200-305 Log in to see 10B8E7
1 ABIN786705 ICC IF Alexa Fluor 546 Mouse IgG1 Log in to see 107D2-08 8
1 ABIN786704 ICC FACS IF Alexa Fluor 488 Mouse IgG1 Log in to see 107D2-08 8

Top referenced anti-IL3RA antibody for Immunocytochemistry

  • Human Monoclonal IL3RA Primary Antibody for ICC, IF - ABIN786705 (8 Publications): Bachmann, Horn, Rudloff, Goren, Holdener, Christen, Darsow, Hunfeld, Koehl, Kind, Pfeilschifter, Kraiczy, Mühl: Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1. in PLoS pathogens 2010 (PubMed)

  • Human Monoclonal IL3RA Primary Antibody for ICC, FACS - ABIN786704 (8 Publications): Brehm, Huenecke, Quaiser, Esser, Bremm, Kloess, Soerensen, Kreyenberg, Seidl, Becker, Mühl, Klingebiel, Bader, Passweg, Schwabe, Koehl: IL-2 stimulated but not unstimulated NK cells induce selective disappearance of peripheral blood cells: concomitant results to a phase I/II study. in PLoS ONE 2011 (PubMed)

  • Human Monoclonal IL3RA Primary Antibody for FACS, ICC - ABIN4899495 (2 Publications): Leskela, Serrano, de la Fuente, Rodríguez-Muñoz, Ramos-Levi, Sampedro-Nuñez, Sánchez-Madrid, González-Amaro, Marazuela: Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells. in PLoS ONE 2015 (PubMed)

  • Human Monoclonal IL3RA Primary Antibody for FACS, ICC - ABIN4899494 (2 Publications): Bovolenta, Pilotti, Mauri, Turci, Ciancianaini, Fisicaro, Bertazzoni, Poli, Casoli et al.: Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte ... in Blood 2001 (PubMed)

  • Human Monoclonal IL3RA Primary Antibody for ICC, FACS - ABIN1724855 (2 Publications): Yue, Fu, Wang, Li, Hu, Fu, Shao: Expression of CD123 and CD114 on the bone marrow cells of patients with myelodysplastic syndrome. in Chinese medical journal 2010 (PubMed)

  • Human Monoclonal IL3RA Primary Antibody for ICC, FACS - ABIN1724866 (2 Publications): Venkataraman, Aguhar, Kreitman, Yuan, Stetler-Stevenson: Characteristic CD103 and CD123 expression pattern defines hairy cell leukemia: usefulness of CD123 and CD103 in the diagnosis of mature B-cell lymphoproliferative disorders. in American journal of clinical pathology 2011 (PubMed)

Similar anti-IL3RA Antibodies

Application / Reactivity Human
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Blocking Reagent (BR) 3 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 9 Antibodies
ELISA 53 Antibodies
ELISA (Detection) 1 Antibodies
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Flow Cytometry (FACS) 145 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 43 Antibodies

General

Antigen Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
Epitope AA 20-305
(16), (15), (13), (11), (11), (10), (8), (8), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(251), (56), (4)
Host Mouse
(160), (99), (41), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL3RA antibody is un-conjugated
(25), (23), (20), (12), (11), (8), (7), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
(183), (149), (61), (46), (31), (26), (26), (24), (14), (13), (9), (8), (8), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL3RA Antibody

Target Details IL3RA Application Details Handling ProductDetails: References for anti-IL3RA antibody (ABIN4899495) Images
Specificity Detects human IL-3 R alpha/CD123 in direct ELISAs and Western blots.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-3 R alpha/CD123, Lys20-Arg305, predicted, Accession # P26951
Clone 32703
Isotype IgG1

Target Details IL3RA

Product Details anti-IL3RA Antibody Application Details Handling ProductDetails: References for anti-IL3RA antibody (ABIN4899495) Images back to top
Antigen
Alternative Name IL-3 R alpha/cd123 (IL3RA Antibody Abstract)
Background Alternative Names: CD123, CD123 antigen, hIL3Ra, hIL-3Ra, IL-3 receptor subunit alpha, IL3R, IL3R alpha, IL-3R subunit alpha, IL3RA, IL-3RA, IL-3R-alpha, IL3RAY, IL3RX, IL3RY, interleukin 3 receptor, alpha (low affinity), interleukin-3 receptor subunit alpha, MGC34174
Gene ID 3563
UniProt P26951
Research Area Signaling, Cytokines, Innate Immunity
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL3RA Antibody Target Details IL3RA Handling ProductDetails: References for anti-IL3RA antibody (ABIN4899495) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 8-25 μg/mL
Neutralization 0.6-1.2 μg/mL
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details ProductDetails: References for anti-IL3RA antibody (ABIN4899495) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-IL3RA antibody (ABIN4899495)

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling Images back to top
Product cited in:

Leskela, Serrano, de la Fuente, Rodríguez-Muñoz, Ramos-Levi, Sampedro-Nuñez, Sánchez-Madrid, González-Amaro, Marazuela: "Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells." in: PLoS ONE, Vol. 10, Issue 4, pp. e0123938, 2015 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Bovolenta, Pilotti, Mauri, Turci, Ciancianaini, Fisicaro, Bertazzoni, Poli, Casoli et al.: "Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte ..." in: Blood, Vol. 99, Issue 1, pp. 224-31, 2001 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Images

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling ProductDetails: References for anti-IL3RA antibody (ABIN4899495) back to top
Supplier Images
Neutralization (Neut) image for anti-IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity)) (AA 20-305) (ABIN4899495) Cell Proliferation Induced by IL-3 and Neutralization by Human IL-3 R alpha/CD123 Ant...
Immunocytochemistry (ICC) image for anti-IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity)) (AA 20-305) (ABIN4899495) IL-3 R alpha/CD123 in Human Peripheral Blood Lymphocytes. IL-3 R alpha/CD123 was dete...
Immunohistochemistry (IHC) image for anti-IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity)) (AA 20-305) (ABIN4899495) IL-3 R alpha/CD123 in Human Tonsil. IL-3 R alpha/CD123 was detected in immersion fixe...