anti-Human IL3RA antibody for Immunocytochemistry

Recommended IL3RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
  • Cyrl
  • CD123
  • IL3R
  • IL3RAY
  • IL3RX
  • IL3RY
  • hIL-3Ra
  • CDw123
  • SUT-1
  • interleukin 3 receptor, alpha
  • interleukin 3 receptor, alpha (low affinity)
  • interleukin 3 receptor, alpha chain
  • Il3ra
  • IL3RA
Epitope
AA 20-305
28
16
15
13
11
10
8
8
6
5
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
284
55
4
Host
Mouse
192
99
40
3
Clonality
Monoclonal
Conjugate
This IL3RA antibody is un-conjugated
26
25
22
13
12
10
9
7
7
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
198
177
77
74
40
40
38
31
29
13
9
8
8
5
5
3
3
3
2
1
1
1
1
1
1
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Catalog No. ABIN4899495
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.040375 ABIN4899494 CyTOF FACS ICC IHC Neut WB Mouse IgG1 AA 20-305 Log in to see 32703 2
26.040375 ABIN1724855 ICC FACS IHC ELISA WB Mouse IgG1 AA 200-305 Log in to see 8E11C5 2
26.040375 ABIN1724866 ICC FACS ELISA WB Mouse IgG1 AA 200-305 Log in to see 10B8E7 2
1 ABIN4880790 ELISA FACS ICC IHC WB Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN4325125 CyTOF ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 8E11C5
1 ABIN4880335 ELISA FACS ICC WB Mouse IgG1 AA 200-305 Log in to see 10B8E7
1 ABIN1174728 ICC IHC IP WB Rabbit IgG AA 19-157 Log in to see Polyclonal
1 ABIN4325124 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 10B8E7
1 ABIN1823498 ICC FACS IHC (p) ELISA WB Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN1905181 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 AA 200-305 Log in to see 10B8E7
1 ABIN5606282 ELISA FACS ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN5606287 ELISA FACS ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN5606285 ELISA FACS ICC IF IHC IHC (p) WB DyLight 550 Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN5606276 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN5606277 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN5606278 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN5606283 ELISA FACS ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN5606286 ELISA FACS ICC IF IHC IHC (p) WB DyLight 650 Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN5606279 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN5606284 ELISA FACS ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 AA 200-305 Log in to see 8E11C5

General

Antigen Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
Epitope AA 20-305
(28), (16), (15), (13), (11), (10), (8), (8), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(284), (55), (4)
Host Mouse
(192), (99), (40), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL3RA antibody is un-conjugated
(26), (25), (22), (13), (12), (10), (9), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
(198), (177), (77), (74), (40), (40), (38), (31), (29), (13), (9), (8), (8), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL3RA Antibody

Target Details IL3RA Application Details Handling ProductDetails: References for anti-IL3RA antibody (ABIN4899495) Images
Specificity Detects human IL-3 R alpha/CD123 in direct ELISAs and Western blots.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-3 R alpha/CD123, Lys20-Arg305, predicted, Accession # P26951
Clone 32703
Isotype IgG1

Target Details IL3RA

Product Details anti-IL3RA Antibody Application Details Handling ProductDetails: References for anti-IL3RA antibody (ABIN4899495) Images back to top
Antigen
Alternative Name IL-3 R alpha/cd123 (IL3RA Antibody Abstract)
Background Alternative Names: CD123, CD123 antigen, hIL3Ra, hIL-3Ra, IL-3 receptor subunit alpha, IL3R, IL3R alpha, IL-3R subunit alpha, IL3RA, IL-3RA, IL-3R-alpha, IL3RAY, IL3RX, IL3RY, interleukin 3 receptor, alpha (low affinity), interleukin-3 receptor subunit alpha, MGC34174
Gene ID 3563
UniProt P26951
Research Area Signaling, Cytokines, Innate Immunity
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL3RA Antibody Target Details IL3RA Handling ProductDetails: References for anti-IL3RA antibody (ABIN4899495) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 8-25 μg/mL
Neutralization 0.6-1.2 μg/mL
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details ProductDetails: References for anti-IL3RA antibody (ABIN4899495) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-IL3RA antibody (ABIN4899495)

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling Images back to top
Product cited in:

Leskela, Serrano, de la Fuente, Rodríguez-Muñoz, Ramos-Levi, Sampedro-Nuñez, Sánchez-Madrid, González-Amaro, Marazuela: "Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells." in: PLoS ONE, Vol. 10, Issue 4, pp. e0123938, 2015 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Bovolenta, Pilotti, Mauri, Turci, Ciancianaini, Fisicaro, Bertazzoni, Poli, Casoli et al.: "Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte ..." in: Blood, Vol. 99, Issue 1, pp. 224-31, 2001 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Images

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling ProductDetails: References for anti-IL3RA antibody (ABIN4899495) back to top
Supplier Images
Neutralization (Neut) image for anti-Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) (AA 20-305) antibody (ABIN4899495) Cell Proliferation Induced by IL-3 and Neutralization by Human IL-3 R alpha/CD123 Ant...
Immunocytochemistry (ICC) image for anti-Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) (AA 20-305) antibody (ABIN4899495) IL-3 R alpha/CD123 in Human Peripheral Blood Lymphocytes. IL-3 R alpha/CD123 was dete...
Immunohistochemistry (IHC) image for anti-Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) (AA 20-305) antibody (ABIN4899495) IL-3 R alpha/CD123 in Human Tonsil. IL-3 R alpha/CD123 was detected in immersion fixe...
Neutralization (Neut) image for anti-Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) (AA 20-305) antibody (ABIN4899495) Cell Proliferation Induced by IL-3 and Neutralization by Human IL-3 R alpha/CD123 Ant...
Immunocytochemistry (ICC) image for anti-Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) (AA 20-305) antibody (ABIN4899495) IL-3 R alpha/CD123 in Human Peripheral Blood Lymphocytes. IL-3 R alpha/CD123 was dete...
Immunohistochemistry (IHC) image for anti-Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) (AA 20-305) antibody (ABIN4899495) IL-3 R alpha/CD123 in Human Tonsil. IL-3 R alpha/CD123 was detected in immersion fixe...