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anti-Non-Human Primate IL3RA antibody for Flow Cytometry

Recommended IL3RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
  • CD123
  • CDw123
  • Cyrl
  • hIL-3Ra
  • IL3R
  • IL3RAY
  • IL3RX
  • IL3RY
  • SUT-1
Reactivity
Human, Non-Human Primate
245
53
3
Host
Mouse
155
99
38
1
Clonality
Monoclonal
Conjugate
This IL3RA antibody is conjugated to EFluor 450
25
22
21
13
10
6
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
178
144
55
41
34
30
29
28
17
11
10
8
4
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN2677437
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Top referenced anti-IL3RA antibody for Flow Cytometry

Similar anti-IL3RA Antibodies

Application / Reactivity Human Non-Human Primate
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Multiplex Assay (MA) 1 Antibodies
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Immunohistochemistry (IHC) 43 Antibodies
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Immunofluorescence (IF) 16 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunochromatography (IC) 3 Antibodies
Functional Studies (Func) 1 Antibodies
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General

Antigen Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
Reactivity Human, Non-Human Primate
(245), (53), (3)
Host Mouse
(155), (99), (38), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL3RA antibody is conjugated to EFluor 450
(25), (22), (21), (13), (10), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(178), (144), (55), (41), (34), (30), (29), (28), (17), (11), (10), (8), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL3RA Antibody

Target Details IL3RA Application Details Handling References for anti-IL3RA Antibody (ABIN2677437) Images
Specificity This 7G3 monoclonal antibody reacts with human and non-human primate CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (the common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. In addition, this molecule can be detected on CD34+CD38- hematopoietic stem cells and acute myeloid leukemic (AML) cells.

The 7G3 antibody has been demonstrated to inhibit IL-3-mediated cell proliferation and homing of AML leukemic stem cells. Crossblocking studies suggest that 7G3 recognizes a different epitope from 6H6.

The 7G3 antibody has been reported to crossreact to chimpanzee, cynomologous monkey, olive baboon, pigtailed macaque, rhesus macaque, and sooty mangabey.
Characteristics This 7G3 monoclonal antibody reacts with human and non-human primate CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (the common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. In addition, this molecule can be detected on CD34+CD38- hematopoietic stem cells and acute myeloid leukemic (AML) cells. The 7G3 antibody has been demonstrated to inhibit IL-3-mediated cell proliferation and homing of AML leukemic stem cells. Crossblocking studies suggest that 7G3 recognizes a different epitope from 6H6. The 7G3 antibody has been reported to crossreact to chimpanzee, cynomologous monkey, olive baboon, pigtailed macaque, rhesus macaque, and sooty mangabey.
Synonyms: Synonyms: Interleukin-3 Receptor alpha, IL-3Ra
Clone 7G3
Isotype IgG2a kappa

Target Details IL3RA

Product Details anti-IL3RA Antibody Application Details Handling References for anti-IL3RA Antibody (ABIN2677437) Images back to top
Antigen
Alternative Name CD123 (IL3RA Antibody Abstract)
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL3RA Antibody Target Details IL3RA Handling References for anti-IL3RA Antibody (ABIN2677437) Images back to top
Application Notes Applications reported: This 7G3 antibody has been reported for use in flow cytometric analysis.

Applications tested: This 7G3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. eFluor® 450 is a replacement for Pacific Blue®. eFluor® 450 emits at 456 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Restrictions For Research Use only

Handling

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details References for anti-IL3RA Antibody (ABIN2677437) Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

References for anti-IL3RA Antibody (ABIN2677437)

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling Images back to top
Product cited in:

Van Brussel, Van Vré, De Meyer, Vrints, Bosmans, Bult: "Expression of dendritic cell markers CD11c/BDCA-1 and CD123/BDCA-2 in coronary artery disease upon activation in whole blood." in: Journal of immunological methods, Vol. 362, Issue 1-2, pp. 168-75, 2010

Images

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling References for anti-IL3RA Antibody (ABIN2677437) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity))  (EFluor 450) (ABIN2677437) anti-Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) antibody (EFluor 450)