|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||1 Antibodies||3 Antibodies|
|Western Blotting (WB)||3 Antibodies||13 Antibodies|
|ELISA||8 Antibodies||52 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Flow Cytometry (FACS)||37 Antibodies|
|Immunocytochemistry (ICC)||16 Antibodies|
|Immunofluorescence (IF)||15 Antibodies|
|Immunohistochemistry (IHC)||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Blocking Reagent (BR)||3 Antibodies|
|Dot Blot (DB)||10 Antibodies|
|Antigen||Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This IL3RA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-IL3RA AntibodyTarget Details IL3RA Application Details Handling Images
|Specificity||This antibody detects endogenous levels of IL-3R protein. (region surrounding Phe587)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography using epitope-specific immunogen|
Target Details IL3RAProduct Details anti-IL3RA Antibody Application Details Handling Images back to top
|Alternative Name||CD123 / IL3RA (IL3RA Antibody Abstract)|
|Background||Interleukin 3 (IL3), originally called Colony Forming Unit-Stimulating Activity (CFU-SA) is a multifunctional cytokine produced by activated T lymphocytes or mast cells. IL3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. It induces colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar suspended bone marrow cells. Interleukin 3 binds specifically and with high affinity to cell-surface receptors. Interleukin 3 receptors are members of the cytokine receptor superfamily. The IL3 receptor complex contains two distinct cytokine receptor subunits, ligand binding IL3 Ralpha and IL3 Rbeta. Recombinant human IL3 sRalpha (286 amino acid residue) has a predicted molecular mass of approximately 33 kDa. The alpha subunit alone binds IL3 with low affinity. The beta subunit does not bind IL3 by itself but is required for the high-affinity binding of IL3 to the heterodimeric receptor complex. The alpha subunit is also a component of the high-affinity receptor complex for IL5 and GMCSF.Synonyms: IL-3R-alpha, IL3 receptor alpha, Interleukin-3 receptor subunit alpha|
|Molecular Weight||approx. 44 kDa|
|Research Area||CD Antigens|
Application DetailsProduct Details anti-IL3RA Antibody Target Details IL3RA Handling Images back to top
ELISA: 1/1000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
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