You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) IL4 Receptor antibody for Flow Cytometry

Recommended IL4 Receptor Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 Receptor (IL4R) Antibodies
  • CD124
  • IL-4RA
  • il-4ra
  • IL4
  • Il4r
  • IL4R
  • IL4RA
  • Il4ra
  • zgc:113063
Reactivity
Mouse (Murine)
93
32
7
Host
Rat
54
46
11
6
1
Clonality
Monoclonal
Conjugate
This IL4 Receptor antibody is conjugated to Biotin
6
4
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut), ELISA
62
51
31
15
13
9
7
6
5
4
4
3
3
2
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN2688938
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010482287 ABIN339552 FACS PE Goat IgG Extracellular Domain Log in to see Polyclonal
0.0010482287 ABIN1177312 FACS IP Neut Rat IgG2a, kappa Log in to see mIL4R 8
0.0010482287 ABIN339551 FACS CF Goat IgG Extracellular Domain Log in to see Polyclonal
0.0010482287 ABIN2688941 FACS IP Neut ELISA Rat IgG2a, kappa Log in to see mIL4R 8
0.0010482287 ABIN327343 FACS ELISA WB Goat IgG Extracellular Domain Log in to see Polyclonal
0.0010482287 ABIN2289271 FACS PE Goat IgG Log in to see Polyclonal
0.0010482287 ABIN2289267 FACS ELISA WB Goat IgG Log in to see Polyclonal
0.0010482287 ABIN2289269 FACS Goat IgG Log in to see Polyclonal

Top referenced anti-IL4 Receptor antibody for Flow Cytometry

Similar anti-IL4 Receptor Antibodies

Application / Reactivity Mouse (Murine)
ELISA 16 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Neutralization (Neut) 4 Antibodies
Western Blotting (WB) 17 Antibodies

General

Antigen Interleukin 4 Receptor (IL4R) Antibodies
Reactivity Mouse (Murine)
(93), (32), (7)
Host Rat
(54), (46), (11), (6), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL4 Receptor antibody is conjugated to Biotin
(6), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut), ELISA
(62), (51), (31), (15), (13), (9), (7), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-IL4 Receptor Antibody

Target Details IL4 Receptor Application Details Handling References for anti-IL4 Receptor Antibody (ABIN2688938) Images
Brand BD Pharmingen™
Characteristics The mIL4R-M1 monoclonal antibody specifically binds to CD124 which is also known as the α subunit of the mouse Interleukin-4 Receptor (IL-4Rα). The mouse IL-4Rα is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc, aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4Rα/IL-13Rα, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M1 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4's biological activities. The mIL4R-M1 antibody also recognizes naturally-occurring, soluble truncated forms of IL-4Rα (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4Rα domain or from differential mRNAsplicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block™ (Cat. No. 553142) to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 (0.125 μg/10^6 cells) followed by streptavidin phycoerythrin (0.015 μg, Cat. No. 554061) and FITC anti-mouse B220 (0.06 μg, Cat. No. 553088) and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells (with the light-scattering characteristics of viable lymphocytes) are shown in the two-color dot plot (right panel). Staining with the biotinylated mIL4R-M1 antibody (right panel) is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody (0.125 μg, Cat. No. 553928) that is shown in the left panel.

BD Pharmingen™ Biotin Rat Anti-Mouse CD124 - Biotin - Clone mIL4R-M1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse CTLL-19.4 T Cell Line
Clone MIL4R
Isotype IgG2a, kappa

Target Details IL4 Receptor

Product Details anti-IL4 Receptor Antibody Application Details Handling References for anti-IL4 Receptor Antibody (ABIN2688938) Images back to top
Antigen
Alternative Name CD124 (IL4R Antibody Abstract)
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Handling References for anti-IL4 Receptor Antibody (ABIN2688938) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details References for anti-IL4 Receptor Antibody (ABIN2688938) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL4 Receptor Antibody (ABIN2688938)

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details Handling Images back to top
Product cited in:

Hassuneh, Nagarkatti, Nagarkatti: "Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin." in: Blood, Vol. 89, Issue 2, pp. 610-20, 1997

Sempowski, Beckmann, Derdak, Phipps: "Subsets of murine lung fibroblasts express membrane-bound and soluble IL-4 receptors. Role of IL-4 in enhancing fibroblast proliferation and collagen synthesis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3606-14, 1994

Chilton, Fernandez-Botran: "Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5907-17, 1994

Feldman, Ruhl, Bickel, Finbloom, Pluznik: "Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6." in: Blood, Vol. 78, Issue 7, pp. 1678-84, 1991