anti-Guinea Pig IL5 antibody for Flow Cytometry

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • IL5
  • Il-5
  • IL-5
  • TRF
  • EDF
  • Pro-TRH
  • interleukin 5
  • Interleukin 5
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5-like
  • thyrotropin-releasing hormone
  • IL5
  • Il5
  • IL-5
  • LOC100358075
  • TRH
Reactivity
Guinea Pig, Human, Mouse (Murine)
210
147
27
19
17
3
2
2
1
1
1
Host
Rat
132
103
41
2
Clonality
Monoclonal
Conjugate
This IL5 antibody is un-conjugated
39
15
9
9
8
8
7
7
7
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
125
125
125
25
23
13
11
10
10
9
8
8
7
6
6
6
6
4
2
2
1
1
Supplier
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Catalog No. ABIN118519
$ 731.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2475029 FACS ELISA WB Rat IgG1 Log in to see TRFK5 4
1 ABIN254410 FACS ELISA WB Rat IgG1 Log in to see TRFK5
1 ABIN218125 FACS ELISA WB Rat IgG1 Log in to see
1 ABIN217640 FACS ELISA WB Rat IgG1 Log in to see TRFK5
1 ABIN4325322 ELISA FACS WB PerCP Rat IgG1 Log in to see TRFK5
1 ABIN4325320 ELISA FACS WB DyLight 350 Rat IgG1 Log in to see TRFK5
1 ABIN4325328 FACS PE Rat IgG1 Log in to see TRFK5
1 ABIN4325327 ELISA FACS WB DyLight 680 Rat IgG1 Log in to see TRFK5
1 ABIN4325324 ELISA FACS WB DyLight 755 Rat IgG1 Log in to see TRFK5
1 ABIN4325343 ELISA FACS WB Alexa Fluor 405 Rat IgG1 Log in to see TRFK5
1 ABIN4325344 ELISA FACS WB Alexa Fluor 488 Rat IgG1 Log in to see TRFK5
1 ABIN4325323 FACS Allophycocyanin Rat IgG1 Log in to see TRFK5
1 ABIN4325321 ELISA FACS WB DyLight 405 Rat IgG1 Log in to see TRFK5
1 ABIN4325345 ELISA FACS WB Alexa Fluor 647 Rat IgG1 Log in to see TRFK5
1 ABIN4325346 ELISA FACS WB Alexa Fluor 700 Rat IgG1 Log in to see TRFK5
1 ABIN4325325 ELISA FACS WB DyLight 488 Rat IgG1 Log in to see TRFK5

General

Antigen Interleukin 5 (IL5) Antibodies
Reactivity Guinea Pig, Human, Mouse (Murine)
(210), (147), (27), (19), (17), (3), (2), (2), (1), (1), (1)
Host Rat
(132), (103), (41), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL5 antibody is un-conjugated
(39), (15), (9), (9), (8), (8), (7), (7), (7), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
(125), (125), (125), (25), (23), (13), (11), (10), (10), (9), (8), (8), (7), (6), (6), (6), (6), (4), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 antibody (ABIN118519) Images
Specificity This antibody is specific for Interleukin 5 (IL-5). Clone TRFK5 may be used to neutralise IL-5 bioactivity in a functional assay (1,2).
Purification Affinity chromatography on Protein G
Immunogen Purified natural murine IL-5.
Clone TRFK5
Isotype IgG1

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 antibody (ABIN118519) Images back to top
Antigen
Alternative Name Interleukin-5 / IL5 (IL5 Antibody Abstract)
Background IL-5 is a T cell derived haematopoietic growth factor, which stimulates eosinophil differentiation and activation.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF
Gene ID 3567
NCBI Accession NP_000870
UniProt P05113
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 antibody (ABIN118519) Images back to top
Application Notes Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: This antibody may be used in combination with clone TRFK4 (ABIN181863) in ELISA and ELISPOT assays. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 antibody (ABIN118519) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL5 antibody (ABIN118519)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Background publications

Akdis, Akdis, Blesken, Wymann, Alkan, Mueller, Blaser: "Epitope-specific T cell tolerance to phospholipase A2 in bee venom immunotherapy and recovery by IL-2 and IL-15 in vitro." in: The Journal of clinical investigation, Vol. 98, Issue 7, pp. 1676-83, 1996

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Betz, Fox: "Regulation and development of cytochrome c-specific IL-4-producing T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 4, pp. 1046-52, 1990

Schumacher, OGarra, Shrader, van Kimmenade, Bond, Mosmann, Coffman: "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 5, pp. 1576-81, 1988