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anti-Human IL5 antibody for ELISA (Capture)

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • IL5
  • Il-5
  • IL-5
  • TRF
  • EDF
  • Pro-TRH
  • interleukin 5
  • Interleukin 5
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5-like
  • thyrotropin-releasing hormone
  • IL5
  • Il5
  • IL-5
  • LOC100358075
  • TRH
Reactivity
Human, Mouse (Murine)
166
126
20
16
16
2
1
1
1
1
Host
Rat
122
94
22
2
Clonality
Monoclonal
Conjugate
This IL5 antibody is un-conjugated
40
14
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
ELISA (Capture), Flow Cytometry (FACS), Neutralization (Neut)
118
118
93
21
15
11
11
11
9
9
6
6
6
6
4
4
3
2
2
1
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Catalog No. ABIN4900786
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4900785 ELISA (Capture) FACS Neut Rat IgG1 Log in to see TRFK5 13
1 ABIN371620 ELISA (Capture) ELISA (Detection) EIA Rat IgG2a Log in to see JES1-5A10 3
1 ABIN371619 ELISA (Capture) ELISA (Detection) EIA Biotin Rat IgG2a Log in to see JES1-5A10

Top referenced anti-IL5 antibody for ELISA (Capture)

Similar anti-IL5 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
Blocking Peptide (BP) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
ELISA 54 Antibodies
ELISA (Capture) 2 Antibodies
ELISpot 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 63 Antibodies
Functional Studies (Func) 4 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 2 Antibodies

General

Antigen Interleukin 5 (IL5) Antibodies
Reactivity Human, Mouse (Murine)
(166), (126), (20), (16), (16), (2), (1), (1), (1), (1)
Host Rat
(122), (94), (22), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL5 antibody is un-conjugated
(40), (14), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture), Flow Cytometry (FACS), Neutralization (Neut)
(118), (118), (93), (21), (15), (11), (11), (11), (9), (9), (6), (6), (6), (6), (4), (4), (3), (2), (2), (1)
Pubmed 13 references available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN4900786) Images
Specificity Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 g of the antibody by the LAL method.
Immunogen Mouse T cell clone-derived partially purified recombinant mouse IL-5
Clone TRFK5
Isotype IgG1

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 Antibody (ABIN4900786) Images back to top
Antigen
Alternative Name IL-5 (IL5 Antibody Abstract)
Gene ID 3567
Research Area Virology, Immunology, Cytokines, Cancer, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 Antibody (ABIN4900786) Images back to top
Application Notes Neutralization 0.004-0.015 μg/mL, Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells, CyTOF-reported, ELISA Capture (Matched Antibody Pair) 2-8 μg/mL, ELISA Capture (Matched Antibody Pair) 2-8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 Antibody (ABIN4900786) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 m filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 6 months, -20 to -70 C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-IL5 Antibody (ABIN4900786)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Product cited in:

Zhang, Yin, Zhang, Sun, Yang, Zhang, Salzman, Wang, Liu, Vidyasagar, Zhang, Ju, Okunieff, Zhang: "Response patterns of cytokines/chemokines in two murine strains after irradiation." in: Cytokine, Vol. 58, Issue 2, pp. 169-77, 2012 Method employed by authors: Luminex Assay (LMNX) (Sample species: Mouse (Murine)).

Yamada, Tani, Nakanishi, Taguchi, Arita, Arai: "Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 2, pp. 561-8, 2011 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Whelan: "Evidence that 13-14 di-hydro, 15-keto prostaglandin D(2)-induced airway eosinophilia in guinea-pigs is independent of interleukin-5." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 58, Issue 2, pp. 103-8, 2009 Method employed by authors: In vivo Studies (in vivo) (Sample species: Guinea Pig).

Xavier-Elsas, Santos-Maximiano, Queto, Mendonça-Sales, Joseph, Gaspar-Elsas, Vargaftig: "Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients' lungs through an allergen- and interleukin-5-dependent mechanism." in: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Vol. 37, Issue 1, pp. 29-38, 2007 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Qui, Ferrell, Nolan, Phelps, Tabibiazar, Whitney, Naelfski: "Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines." in: Clinical chemistry, Vol. 53, Issue 11, pp. 2010-2, 2007 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Pfleger, Schloot, ter Veld: "Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays." in: Journal of immunological methods, Vol. 329, Issue 1-2, pp. 214-8, 2007 Method employed by authors: Luminex Assay (LMNX) (Sample species: Human).

Gounni Abdelilah, Wellemans, Agouli, Guenounou, Hamid, Beck, Lamkhioued: "Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines." in: Clinical immunology (Orlando, Fla.), Vol. 120, Issue 2, pp. 220-31, 2006 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Wen, Hogaboam, Gauldie, Kunkel: "Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile." in: The American journal of pathology, Vol. 168, Issue 6, pp. 1940-50, 2006 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Nygaard, Ormstad, Aase, Løvik: "The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node." in: Toxicology, Vol. 206, Issue 2, pp. 181-93, 2004 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Couillin, Maillet, Vargaftig, Jacobs, Paesen, Nuttall, Lefort, Moser, Weston-Davies, Ryffel: "Arthropod-derived histamine-binding protein prevents murine allergic asthma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 5, pp. 3281-6, 2004 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Kobayashi, Iijima, Kita: "Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 11, pp. 5756-63, 2003 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Lukacs, Berlin, Schols, Skerlj, Bridger: "AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity." in: The American journal of pathology, Vol. 160, Issue 4, pp. 1353-60, 2002 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Tomaki, Zhao, Sjöstrand, Lindén, Ichinose, Lötvall: "Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen." in: Pulmonary pharmacology & therapeutics, Vol. 15, Issue 2, pp. 161-8, 2002 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Images

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN4900786) back to top
Supplier Images
Neutralization (Neut) image for anti-IL5 antibody (Interleukin 5) (ABIN4900786) Cell Proliferation Induced by IL-5 and Neutralization by Human/Mouse IL-5 Antibody. R...
Flow Cytometry (FACS) image for anti-IL5 antibody (Interleukin 5) (ABIN4900786) Detection of IL-5 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes ...