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anti-Human IL5 antibody for Immunocytochemistry

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • IL5
  • Il-5
  • IL-5
  • TRF
  • EDF
  • Pro-TRH
  • interleukin 5
  • Interleukin 5
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5-like
  • thyrotropin-releasing hormone
  • IL5
  • Il5
  • IL-5
  • LOC100358075
  • TRH
Epitope
AA 20-134
27
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
173
131
26
19
17
3
2
2
1
1
Host
Mouse
114
101
23
2
Clonality
Monoclonal
Conjugate
This IL5 antibody is un-conjugated
36
14
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
122
122
92
23
22
13
11
10
9
9
9
6
6
6
6
4
4
2
2
1
1
1
Supplier
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Catalog No. ABIN4900611
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1868680 ICC IHC IP WB Rabbit IgG AA 20-134 Log in to see Polyclonal

Top referenced anti-IL5 antibody for Immunocytochemistry

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Application / Reactivity Human
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Cell Culture (CC) 1 Antibodies
ELISA 83 Antibodies
ELISA (Capture) 4 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 68 Antibodies
Functional Studies (Func) 9 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies

General

Antigen Interleukin 5 (IL5) Antibodies
Epitope AA 20-134
(27), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(173), (131), (26), (19), (17), (3), (2), (2), (1), (1)
Host Mouse
(114), (101), (23), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL5 antibody is un-conjugated
(36), (14), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(122), (122), (92), (23), (22), (13), (11), (10), (9), (9), (9), (6), (6), (6), (6), (4), (4), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN4900611) Images
Specificity Detects human IL-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-5 is observed.
Purification Protein A or G purified from ascites
Sterility 0.2 μm filtered
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-5, Ile20-Ser134, Accession # P05113
Clone 9906
Isotype IgG1

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 Antibody (ABIN4900611) Images back to top
Antigen
Alternative Name IL-5 (IL5 Antibody Abstract)
Background Alternative Names: BCDF mu, B-cell differentiation factor I, BCGFII, EDF, Eo-CSF, Eosinophil differentiation factor, IL5, IL-5T-cell replacing factor, interleukin 5 (colony-stimulating factor, eosinophil), interleukin-5, TRF, TRFB cell differentiation factor I
Gene ID 3567
UniProt P05113
Research Area Adaptive Immunity, Cytokines, Innate Immunity, Cancer
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 Antibody (ABIN4900611) Images back to top
Application Notes Western Blot 1 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 Antibody (ABIN4900611) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-IL5 Antibody (ABIN4900611)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Product cited in:

Miller, Ramsdell, Friedman, Cho, Renvall, Broide: "Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema." in: The Journal of allergy and clinical immunology, Vol. 120, Issue 5, pp. 1118-25, 2007 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Sen, Chunsong, Baojun, Linjie, Qun, San, Qiuping, Junyan, Zhang, Jinquan: "Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells." in: Immunology, Vol. 112, Issue 2, pp. 274-89, 2004 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Images

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN4900611) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL5 antibody (Interleukin 5) (AA 20-134) (ABIN4900611) Detection of IL-5 in PMA and Ca2+ ionomycin-treated Human PBMCs by Flow Cytometry. Hu...
Immunocytochemistry (ICC) image for anti-IL5 antibody (Interleukin 5) (AA 20-134) (ABIN4900611) IL-5 in Human Peripheral Blood Lymphocytes. IL-5 was detected in immersion fixed huma...