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anti-Human IL5RA antibody for Western Blotting

Recommended IL5RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 Receptor, alpha (IL5RA) Antibodies
  • CD125
  • CDw125
  • cdw125
  • gpIL-5ra
  • HSIL5R3
  • hsil5r3
  • il5r
  • Il5r
  • IL5R
  • IL5RA
  • MGC86517
Epitope
AA 53-68, N-Term
26
15
15
9
8
5
2
1
1
1
1
1
1
1
1
Reactivity
Human
81
30
21
Host
Rabbit
80
4
Clonality
Polyclonal
Conjugate
This IL5RA antibody is un-conjugated
7
6
5
5
4
4
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
58
44
26
16
11
8
2
2
Supplier
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Catalog No. ABIN3043143
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13029787 ABIN2789551 WB Rabbit N-Term Log in to see Polyclonal
0.13029787 ABIN651428 FACS IHC (p) WB Rabbit Ig AA 49-76, N-Term Log in to see Polyclonal
0.13029787 ABIN2488036 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.13029787 ABIN3187749 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.13029787 ABIN1174731 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 42-182 Log in to see Polyclonal
0.13029787 ABIN2434824 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13029787 ABIN2488037 WB Rabbit IgG N-Term Log in to see Polyclonal
0.13029787 ABIN2489310 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.13029787 ABIN2433197 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13029787 ABIN517033 WB Mouse AA 1-335, full length Log in to see Polyclonal
0.0011766474 ABIN2624147 WB Rabbit IgG AA 53-68 Log in to see Polyclonal
0.0011766474 ABIN1905195 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term, AA 57-87 Log in to see Polyclonal
0.0011766474 ABIN1905196 FACS IHC ELISA WB APC Rabbit IgG N-Term, AA 57-87 Log in to see Polyclonal
0.0011766474 ABIN1905197 FACS IHC ELISA WB Biotin Rabbit IgG N-Term, AA 57-87 Log in to see Polyclonal
0.0011766474 ABIN1905198 FACS IHC ELISA WB FITC Rabbit IgG N-Term, AA 57-87 Log in to see Polyclonal
0.0011766474 ABIN1905199 FACS IHC ELISA WB PE Rabbit IgG N-Term, AA 57-87 Log in to see Polyclonal
0.0011766474 ABIN1905189 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 389-420 Log in to see Polyclonal
0.0011766474 ABIN1905190 ELISA WB APC Rabbit IgG C-Term, AA 389-420 Log in to see Polyclonal
0.0011766474 ABIN1905191 ELISA WB Biotin Rabbit IgG C-Term, AA 389-420 Log in to see Polyclonal
0.0011766474 ABIN1905193 ELISA WB PE Rabbit IgG C-Term, AA 389-420 Log in to see Polyclonal

Similar anti-IL5RA Antibodies

Application / Reactivity Human
ELISA 43 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 26 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Western Blotting (WB) 57 Antibodies

General

Antigen Interleukin 5 Receptor, alpha (IL5RA) Antibodies
Epitope AA 53-68, N-Term
(26), (15), (15), (9), (8), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(81), (30), (21)
Host Rabbit
(80), (4)
Clonality
Polyclonal
Conjugate This IL5RA antibody is un-conjugated
(7), (6), (5), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(58), (44), (26), (16), (11), (8), (2), (2)
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Product Details anti-IL5RA Antibody

Target Details IL5RA Application Details Handling Images
Sequence NPDQEQRNVN LEYQVK
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Interleukin-5 receptor subunit alpha(IL5RA) detection. Tested with WB in Human.
Gene Name: interleukin 5 receptor, alpha
Protein Name: Interleukin-5 receptor subunit alpha(IL-5 receptor subunit alpha/IL-5R subunit alpha/IL-5R-alpha/IL-5RA)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human IL5RA(53-68aa NPDQEQRNVNLEYQVK).
Isotype IgG

Target Details IL5RA

Product Details anti-IL5RA Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL5RA (IL5RA Antibody Abstract)
Background IL5RA(INTERLEUKIN 5 RECEPTOR, ALPHA), also known as CD125, is a subunit of the Interleukin-5 receptor. IL5RA also denotes its human gene. The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor which is composed of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3(IL3), colony stimulating factor 2(CSF2/GM-CSF), and interleukin 5(IL5). The IL5RA gene is mapped to 3p26-p24 by Southern blot analysis of DNA from a panel of mouse-human hybrid somatic cell lines complemented by in situ hybridization. And its Cytogenetic location is 3p26.2. Within this 15-residue stretch of IL5RA, the C-terminal phenylalanine is critical. The binding of this protein to IL5 depends on the beta subunit which is activated by the ligand binding and is required for the biological activities of IL5.
UniProt Q01344
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL5RA Antibody Target Details IL5RA Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-IL5RA Antibody Target Details IL5RA Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-IL5RA Antibody Target Details IL5RA Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL5RA antibody (Interleukin 5 Receptor, alpha) (AA 53-68) (ABIN3043143) anti-Interleukin 5 Receptor, alpha (IL5RA) (AA 53-68), (N-Term) antibody