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|Application / Reactivity||Human|
|Flow Cytometry (FACS)||22 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||29 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Western Blotting (WB)||65 Antibodies|
|Antigen||Interleukin 5 Receptor, alpha (IL5RA) Antibodies|
|Epitope||AA 53-68, N-Term Alternatives|
|Conjugate||This IL5RA antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-IL5RA AntibodyTarget Details IL5RA Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Interleukin-5 receptor subunit alpha(IL5RA) detection. Tested with WB in Human.
Gene Name: interleukin 5 receptor, alpha
Protein Name: Interleukin-5 receptor subunit alpha(IL-5 receptor subunit alpha/IL-5R subunit alpha/IL-5R-alpha/IL-5RA)
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human IL5RA(53-68aa NPDQEQRNVNLEYQVK).|
Target Details IL5RAProduct Details anti-IL5RA Antibody Application Details Handling Images back to top
|Alternative Name||IL5RA (IL5RA Antibody Abstract)|
|Background||IL5RA(INTERLEUKIN 5 RECEPTOR, ALPHA), also known as CD125, is a subunit of the Interleukin-5 receptor. IL5RA also denotes its human gene. The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor which is composed of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3(IL3), colony stimulating factor 2(CSF2/GM-CSF), and interleukin 5(IL5). The IL5RA gene is mapped to 3p26-p24 by Southern blot analysis of DNA from a panel of mouse-human hybrid somatic cell lines complemented by in situ hybridization. And its Cytogenetic location is 3p26.2. Within this 15-residue stretch of IL5RA, the C-terminal phenylalanine is critical. The binding of this protein to IL5 depends on the beta subunit which is activated by the ligand binding and is required for the biological activities of IL5.|
Application DetailsProduct Details anti-IL5RA Antibody Target Details IL5RA Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL5RA Antibody Target Details IL5RA Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-IL5RA Antibody Target Details IL5RA Application Details Handling back to top