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|Antigen||Interleukin 5 Receptor, alpha (IL5RA) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This IL5RA antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-IL5RA AntibodyTarget Details IL5RA Application Details Handling Images
|Specificity||This antibody detects endogenous levels of IL-5Ralpha protein. (region surrounding Lys68)|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse.|
|Purification||Affinity Chromatography using epitope-specific immunogen|
Target Details IL5RAProduct Details anti-IL5RA Antibody Application Details Handling Images back to top
|Alternative Name||CD125 / IL5RA (IL5RA Antibody Abstract)|
|Background||Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cellreplacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membraneproximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear protooncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2. Alternative splicing of the IL-5Ralpha gene produces several isoforms, including a membraneanchored isoform and a soluble isoform. The soluble isoform competes with IL-5 for binding to IL-5R and inhibits IL-5-mediated receptor activation and inflammatory mediator production, and, therefore, may be useful in treating asthma.Synonyms: IL-5R-alpha, IL5 Receptor alpha, Interleukin-5 receptor subunit alpha|
Application DetailsProduct Details anti-IL5RA Antibody Target Details IL5RA Handling Images back to top
Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL5RA Antibody Target Details IL5RA Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-IL5RA Antibody Target Details IL5RA Application Details Handling back to top