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anti-Human IL7R antibody for Immunofluorescence

Recommended IL7R Antibody (supplied by: Log in to see )

Antigen
Interleukin 7 Receptor (IL7R) Antibodies
  • CD127
  • CDW127
  • IL-7R-alpha
  • IL-7RA
  • IL-7Ralpha
  • IL7R
  • IL7RA
  • ILRA
Epitope
pTyr449
24
18
13
13
11
6
6
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
227
188
28
3
Host
Rabbit
153
105
98
13
2
Clonality
Polyclonal
Conjugate
This IL7R antibody is un-conjugated
26
24
22
16
15
14
10
9
9
7
7
6
6
5
5
5
5
5
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
170
157
65
65
52
22
12
12
12
9
5
4
4
4
3
3
3
2
2
1
1
Supplier
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Catalog No. ABIN1531668
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN2490722 IF ELISA WB Rabbit IgG pTyr449 Log in to see Polyclonal
0.32040194 ABIN2404126 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN2659948 FACS IF IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see A019D5 6
0.0010840179 ABIN2659949 FACS IF IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see A019D5 6
0.0010840179 ABIN3182449 ELISA IF WB Rabbit IgG pTyr449 Log in to see Polyclonal
0.0010840179 ABIN2888177 IF Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN2697673 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN2989547 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN786859 ICC IF Alexa Fluor 546 Mouse IgG1 Log in to see R34-34 39
0.0010840179 ABIN1854927 ICC IF WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010840179 ABIN1577228 IF ELISA WB Rabbit IgG pTyr449 Log in to see Polyclonal

Top referenced anti-IL7R antibody for Immunofluorescence

  • Human Monoclonal IL7R Primary Antibody for ICC, IF - ABIN786859 (39 Publications): Bernink, Peters, Munneke, te Velde, Meijer, Weijer, Hreggvidsdottir, Heinsbroek, Legrand, Buskens, Bemelman, Mjösberg, Spits: Human type 1 innate lymphoid cells accumulate in inflamed mucosal tissues. in Nature immunology 2013 (PubMed)

  • Human Monoclonal IL7R Primary Antibody for FACS, IF - ABIN2659948 (6 Publications): Huster, Busch, Schiemann, Linkemann, Kerksiek, Wagner, Busch: Selective expression of IL-7 receptor on memory T cells identifies early CD40L-dependent generation of distinct CD8+ memory T cell subsets. in Proceedings of the National Academy of Sciences of the United States of America 2004 (PubMed)

  • Human Monoclonal IL7R Primary Antibody for FACS, IF - ABIN2659949 (6 Publications): Liu, Putnam, Xu-Yu, Szot, Lee, Zhu, Gottlieb, Kapranov, Gingeras, Fazekas de St Groth, Clayberger, Soper, Ziegler, Bluestone: CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells. in The Journal of experimental medicine 2006 (PubMed)

Similar anti-IL7R Antibodies

Application / Reactivity Mouse (Murine) Human
Biochemical Assay (BCA) 1 Antibodies 2 Antibodies
Blocking Reagent (BR) 3 Antibodies
ELISA 38 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 4 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 87 Antibodies
Functional Studies (Func) 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Intracellular Flow Cytometry (ICFC) 12 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Western Blotting (WB) 95 Antibodies

General

Antigen Interleukin 7 Receptor (IL7R) Antibodies
Epitope pTyr449
(24), (18), (13), (13), (11), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(227), (188), (28), (3)
Host Rabbit
(153), (105), (98), (13), (2)
Clonality
Polyclonal
Conjugate This IL7R antibody is un-conjugated
(26), (24), (22), (16), (15), (14), (10), (9), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(170), (157), (65), (65), (52), (22), (12), (12), (12), (9), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
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Product Details anti-IL7R Antibody

Target Details IL7R Application Details Handling Images
Specificity IL-7R/CD127 (Phospho-Tyr449) Antibody detects endogenous levels of IL-7R/CD127 only when phosphorylated at Tyr449.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IL-7R/CD127 around the phosphorylation site of Tyr449.
Isotype IgG

Target Details IL7R

Product Details anti-IL7R Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-7R/CD127 (IL7R Antibody Abstract)
Background Molecular weight of target protein: 51 kDa
Synonyms: CD127 antigen, CDw127, IL-7R alpha, IL7RA, interleukin-7 receptor alpha chain
Molecular Weight 51 kDa
Gene ID 3575
OMIM 126200
UniProt P16871
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL7R Antibody Target Details IL7R Handling Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.591742, Hs.635723 (NCBI Gene Symbol: IL7RA)

Restrictions For Research Use only

Handling

Product Details anti-IL7R Antibody Target Details IL7R Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-IL7R Antibody Target Details IL7R Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL7R antibody (Interleukin 7 Receptor) (pTyr449) (ABIN1531668) Western blot analysis of extracts from COLO205 cells, using IL-7R/CD127 (Phospho-Tyr4...
Immunofluorescence (IF) image for anti-IL7R antibody (Interleukin 7 Receptor) (pTyr449) (ABIN1531668) Immunofluorescence analysis of HuvEc cells, using IL-7R/CD127 (Phospho-Tyr449) Antibo...